A convenient strategy to purify oligonucleotides (ONs) synthesized by solid phase synthesis on an automatic DNA/RNA synthesizer was described. By attaching a photocleavable azide linker as the last phosphoramidite unit in the ON synthesis, only the desired full-length sequence was ‘caught’ on a controlled pore glass (CPG) resin possessing an aza-dimethoxycyclooctyne (DIBAC) derivative. Washing the resulting CPG resin to remove all unbounded species, the subsequent photoirradiation allowed the pure ONs to be ‘released’ without leaving any chemical modifications on native ON structure or chemical reagents from the solid phase ON synthesis.

描述了一种纯化策略，用于纯化在自动DNA / RNA合成仪上通过固相合成合成的寡核苷酸（ON）。通过将光可裂解的叠氮基连接基连接为ON合成中的最后一个亚磷酰胺单元，只有所需的全长序列被“捕获”在具有氮杂二甲氧基环辛炔（DIBAC）衍生物的可控孔玻璃（CPG）树脂上。洗涤所得的CPG树脂以除去所有未结合的物质，随后的光辐照使纯ON得以“释放”，而对天然ON结构或固相ON合成中的化学试剂没有任何化学修饰。