Post-surgery mesh infections are one of the most common complications of hernia repair at the interface of implant materials and tissue, because high doses of antimicrobial agents are toxic and low doses of antibacterial agents are ineffective, with no good clinical solution currently available. To reduce the infection rates after mesh implantation, we designed a “one-pot synthesized” mesoporous silica nanoplatform consisting of levofloxacin (Lev) and silver (Lev@MSN@Ag) composites with poly-L-lactide (PLLA) electrospun fibrous membranes via blending electrospinning. With advances in the combined antibacterial agents Lev and Ag at a low dosage for the treatment of drug-resistant bacterial infections (28 μg mL⁻¹ Lev and 12 μg mL⁻¹ Ag) with a concentration of 5 × 10⁵ CFU mL⁻¹, the composite electrospun fibers act as a carrier for drug-loading and have an antibacterial effect over 8 weeks. Lev@MSN@Ag–PLLA fibers showed a superior antibacterial effect on drug resistant strains in the in vitro test at low doses of antibacterial agents. Further, the in vivo study showed that Lev@MSN@Ag–PLLA fibers significantly inhibited bacterial growth and infection over 8 weeks through the combined effect of low dosage antibacterial drugs. In conclusion, the Lev@MSN@Ag–PLLA fibers provided an advanced combined antibacterial nanoplatform of low dosage for the treatment of drug-resistant bacterial infections.

中文翻译：

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左氧氟沙星/银共负载电纺纤维膜的体外和体内联合抗菌作用

手术后网状感染是在植入物材料和组织的界面处疝气修复的最常见并发症之一，因为高剂量的抗菌剂是有毒的，而低剂量的抗菌剂是无效的，目前尚无良好的临床解决方案。为了降低网状植入后的感染率，我们设计了一种“一锅合成”介孔二氧化硅纳米平台，该平台由左氧氟沙星（Lev）和银（Lev @ MSN @ Ag）复合物与聚L-丙交酯（PLLA）电纺丝纤维膜组成，通过混合电纺。随着低剂量Lev和Ag组合抗菌剂的发展，用于治疗耐药细菌感染（28μgmL ^-1 Lev和12μgmL ^-1Ag）的浓度为5×10 ⁵ CFU mL ^-1，复合电纺纤维可作为载药载体，并在8周内具有抗菌作用。Lev @ MSN @ Ag–PLLA纤维在体外试验中以低剂量的抗菌剂对耐药菌株表现出优异的抗菌作用。此外，体内研究表明，通过低剂量抗菌药物的联合作用，Lev @ MSN @ Ag-PLLA纤维可在8周内显着抑制细菌生长和感染。总之，Lev @ MSN @ Ag-PLLA纤维提供了一种先进的低剂量组合抗菌纳米平台，用于治疗耐药细菌感染。