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Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring
Nature Chemical Biology ( IF 14.8 ) Pub Date : 2017-08-07 00:00:00 , DOI: 10.1038/nchembio.2448
Li Zha , Yindi Jiang , Matthew T Henke , Matthew R Wilson , Jennifer X Wang , Neil L Kelleher , Emily P Balskus

Despite containing an α-amino acid, the versatile cofactor S-adenosylmethionine (SAM) is not a known building block for nonribosomal peptide synthetase (NRPS) assembly lines. Here we report an unusual NRPS module from colibactin biosynthesis that uses SAM for amide bond formation and subsequent cyclopropanation. Our findings showcase a new use for SAM and reveal a novel biosynthetic route to a functional group that likely mediates colibactin's genotoxicity.

中文翻译:

Colibactin生产线酶使用S-腺苷甲硫氨酸构建环丙烷环

尽管含有α-氨基酸,但通用的辅因子S-腺苷甲硫氨酸(SAM)并不是非核糖体肽合成酶(NRPS)装配线的已知构件。在这里,我们报告了一种由大肠杆菌合成的不寻常的NRPS模块,该模块使用SAM形成酰胺键并随后进行环丙烷化。我们的发现展示了SAM的新用途,并揭示了一种新的生物合成途径,该途径可能介导了大肠杆菌素的基因毒性。
更新日期:2017-09-20
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