RNase H is a non-specific endonuclease which degrades selectively the RNA strand in DNA/RNA duplexes. We demonstrate in the present study that 5′-boronic acid modified oligonucleotides hybridized to a RNA target sequence converts RNase H to an inactivated enzyme complex. The dynamic formation of a boronate ester upon addition of a diol moiety disrupts the enzyme-inhibitor complex and reactivates RNase H. Moreover, we show that reactivation of RNase H function can also be engineered through short RNA trimers inputs that fashion RNase H from a non-specific DNA-guided enzyme into an informational and programmable RNA-guided one. Examples of programmable RNA recognition and cleavage illustrate the potential of this new stimuli-responsive system.

中文翻译：

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使用硼酸酯形成的RNA定向的RNase H活性的开关

RNase H是一种非特异性核酸内切酶，可选择性降解DNA / RNA双链体中的RNA链。我们在本研究中证明，与RNA靶序列杂交的5'-硼酸修饰的寡核苷酸可将RNase H转化为灭活的酶复合物。加入二醇部分后，硼酸酯的动态形成会破坏酶抑制剂复合物并重新激活RNaseH。此外，我们显示，也可以通过短RNA三聚体输入来改造RNase H功能，从而从非特异的DNA引导的酶转变为信息性且可编程的RNA引导的酶。可编程RNA识别和切割的例子说明了这种新的刺激反应系统的潜力。