Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2017-09-19 , DOI: 10.1016/j.snb.2017.09.111 Mei Chen , Yuanyuan Wang , Huilan Su , Li Mao , Xinni Jiang , Tao Zhang , Xiaozhen Dai
Many studies have confirmed that CYFRA21-1 DNA is a sensitive and specific marker for non-small cell lung cancer (NSCLC). Hence, there is an urgent need to explore new strategies for early diagnosis of CYFRA21-1 DNA in the clinic. Here, we report a three-dimensional electrochemical DNA biosensor based on 3D graphene functionalized with Ag nanoparticles for sensitive DNA detection. The highly conductive nanocomposite layer was characterized by using scanning and transmission electron microscopy, contact angle analysis, and cyclic voltammetry. The 3D GF/AgNPs provide a favorable microenvironment to retain the bioactivity of immobilized probe ssDNA and effectively promote electron transfer owing to their excellent biocompatibility and good conductivity. Under optimal conditions, the biosensor could detect target DNA down to 1.0 × 10−14 M with high sensitivity, and the peak currents were linear with the logarithm of the concentrations of target DNA from 1.0 × 10−14 to 1.0 × 10−7 M. Furthermore, in clinical trials, the three-dimensional biosensing system detected CYFRA21-1 DNA in real lung cancer samples with satisfactory results. Our research suggests that this newly developed 3D electrochemical biosensor is a promising method for potential use in the early diagnosis of lung cancer patients.
中文翻译:
基于3D石墨烯-Ag纳米粒子的三维电化学DNA生物传感器用于非小细胞肺癌中CYFRA21-1的灵敏检测
许多研究已经证实CYFRA21-1 DNA是非小细胞肺癌(NSCLC)的敏感和特异性标志物。因此,迫切需要探索CYFRA21-1早期诊断的新策略。诊所中的DNA。在这里,我们报告了基于3D石墨烯的3D电化学DNA生物传感器,该3D石墨烯被Ag纳米粒子官能化以用于敏感的DNA检测。通过使用扫描和透射电子显微镜,接触角分析和循环伏安法对高导电纳米复合材料层进行了表征。3D GF / AgNPs具有出色的生物相容性和良好的电导率,从而提供了良好的微环境,可保留固定化探针ssDNA的生物活性并有效地促进电子转移。在最佳条件下,生物传感器可以 高灵敏度检测低至1.0×10 -14 M的目标DNA ,并且峰值电流与目标DNA浓度从1.0×10 -14到1.0×10 -7的对数呈线性关系 M.此外,在临床试验中,三维生物传感系统在真实的肺癌样本中检测到CYFRA21-1 DNA,结果令人满意。我们的研究表明,这种新开发的3D电化学生物传感器是一种有前途的方法,可用于肺癌患者的早期诊断。