Amending feed water with biocide is one of the strategy conventionally used to control biofouling in membrane-based water treatment systems. In this study, the impacts of two biocides, monochloramine (MCA) and hydrogen peroxide (H₂O₂), on the bacterial community in wastewater samples were investigated at equivalent biocidal efficiency levels. Viable bacterial numbers were determined before and after treatment for 10 min and 60 min using both culture-dependent heterotrophic plate count (HPC) and culture-independent propidium monoazide (PMA)-droplet digital PCR (ddPCR). Shifts of the live bacterial diversity were studied using high-throughput sequencing of 16S rRNA genes and followed by bioinformatics analysis. At the genus level, MCA treatment increased the relative abundance of Mycobacterium, Pseudomonas, Sphingomonas, Clostridium, Streptococcus, Undibacterium, Chryseobacterium and Cloacibacterium, while decreasing Arcobacter, Nitrospira and Sphingobium. H₂O₂ treatment increased the relative abundance of Anaerolinea and Filimonas, and diminished Denitratisoma and Thauera. The findings of this study suggest a combination of different types of biocide may be the most efficient strategy for biofouling mitigation and increasing membrane treatment efficiency.

中文翻译：

---

一氯胺和过氧化氢对生物处理废水中细菌群落迁移的影响

用杀生物剂修正给水是常规用于控制基于膜的水处理系统中生物污垢的策略之一。在这项研究中，以等效的杀菌效率水平研究了两种杀菌剂一氯胺（MCA）和过氧化氢（H ₂ O ₂）对废水样品中细菌群落的影响。使用培养依赖性异养平板计数（HPC）和培养无关的单叠氮化丙锭（PMA）-液滴数字PCR（ddPCR）来确定在处理10分钟和60分钟之前和之后的活细菌数。研究人员利用16S rRNA基因的高通量测序研究了活细菌多样性的变化，然后进行了生物信息学分析。在属水平上，MCA治疗可增加分枝杆菌，假单胞菌，鞘氨醇单胞菌，梭状芽孢杆菌，链球菌，双歧杆菌，Chryseobacterium和Cloacibacterium，同时减少了Arcobacter，Nitrospira和Sphingobium。ħ ₂ ö ₂处理增加的相对丰度Anaerolinea和Filimonas，和减少Denitratisoma和Thauera。 这项研究的结果表明，不同类型的杀菌剂的组合可能是减轻生物污染和提高膜处理效率的最有效策略。