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Pyruvate-Kinase-Coupled Glycosyltransferase Assays: Limitations, Struggles and Problem Resolution
ChemBioChem ( IF 3.2 ) Pub Date : 2017-09-19 07:15:41 , DOI: 10.1002/cbic.201700326
Huixiao Fu 1 , Weidong Pan 2 , Stéphane P. Vincent 1
Affiliation  

No longer out of WaaC: Pyruvate-kinase-coupled (PK-coupled) glycosyltransferase assays can be troublesome when the compound under study inhibits or activates the PK. Thanks to the development of new kinetic protocols, potent inhibitors of the heptosyltransferase WaaC were discovered and characterized even when the standard PK-coupled procedures failed.

中文翻译:

丙酮酸激酶偶联的糖基转移酶测定:局限性,斗争和问题解决

不再脱离WaaC:当所研究的化合物抑制或激活PK时,丙酮酸激酶偶联(PK偶联)的糖基转移酶测定可能会很麻烦。由于开发了新的动力学方案,即使标准的PK偶联程序失败了,也发现并表征了庚基转移酶WaaC的有效抑制剂。
更新日期:2017-09-19
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