Recently, there has been much interest in the chirality-dependent cell affinity to enantiomorphous nanomaterials (NMs), since, at the nanoscale level, enantiomers of (bio)molecules have different effects on cell behaviors. In this respect, this study used enantiomorphous NMs with which to generate the Janus nanocomposite (NC) hydrogels as multifunctional biomaterials for studying chirality-dependent cell adhesion and cell migration. These Janus NC hydrogels possess two enantiomorphous NC hydrogels, in which the different halves of the hydrogel contain the opposite enantiomers of a biopolymer functionalized nanomaterials. Thus, the enantiomers contact each other only at the midline of the hydrogel but are otherwise separated, yet they are present in the same system. This advanced system allows us to simultaneously study the impact that each enantiomer of a biopolymer has on cell behavior under the same reaction conditions, at the same time, and using only a single biomaterial. Our results show that cells have higher affinity for and migrate toward the part of the Janus NC hydrogel containing the biopolymer enantiomer that the cells prefer.

中文翻译：

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Janus纳米复合水凝胶用于手性依赖细胞的粘附和迁移。

近来，对手性依赖的细胞与对映体纳米材料（NMs）有很大的兴趣，因为在纳米级，（生物）分子的对映异构体对细胞行为具有不同的影响。在这方面，这项研究使用了能产生Janus纳米复合物（NC）水凝胶的对映体NMs作为多功能生物材料，用于研究手性依赖的细胞粘附和细胞迁移。这些Janus NC水凝胶具有两种对映体NC水凝胶，其中水凝胶的不同部分包含生物聚合物功能化纳米材料的相反对映异构体。因此，对映异构体仅在水凝胶的中线彼此接触，但是以其他方式分离，但是它们存在于同一系统中。这种先进的系统使我们能够同时研究生物聚合物的每种对映异构体在相同反应条件下，同时且仅使用一种生物材料对细胞行为的影响。我们的结果表明，细胞对Janus NC水凝胶的部分具有更高的亲和力并向其中迁移，该部分含有细胞偏爱的生物聚合物对映体。