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Towards a high throughput impedimetric screening of chemosensitivity of cancer cells suspended in hydrogel and cultured in a paper substrate
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2017-09-19 , DOI: 10.1016/j.bios.2017.09.029
Kin Fong Lei , Tai-Kun Liu , Ngan-Ming Tsang

In order to achieve high predictive value of cell chemosensitivity test for clinical efficacy, cancer cells were suggested to be encapsulated and cultured in hydrogel to mimic the natural microenvironment of tumors. However, handling 3D cells/hydrogel culture construct is tedious and cellular response is difficult to be quantitatively analyzed. In the current study, a novel platform for conducting 3D cell culture and analyzing cell viability has been developed towards a high throughput drug screening. Cells encapsulated in the hydrogel were cultured in the microwells of a paper substrate. The substrate was then immersed in the culture medium containing drug for 2 days. At 24 and 48 h during the culture course, the paper substrate was placed on the measurement electrodes for conducting the impedance measurement in order to quantify the cell viability in the hydrogel. Cell viability of two human hepatoma cell lines (Huh7 and Hep-G2) was quantitatively investigated under the treatment of two drugs (doxorubicin and etoposide). The results represented by IC50 values revealed that Huh7 cells had a higher drug resistance than Hep-G2 cells and doxorubicin had a higher efficacy than etoposide for treating hepatocellular carcinoma. The current work has demonstrated a high throughput, convenient, and quantitative platform for the investigation of chemosensitivity of cells cultured in the 3D environment.



中文翻译:

旨在高阻滞筛选悬浮在水凝胶中并培养在纸质基质中的癌细胞的化学敏感性

为了对细胞化学敏感性测试的临床疗效具有较高的预测价值,建议将癌细胞封装并在水凝胶中培养以模拟肿瘤的自然微环境。但是,处理3D细胞/水凝胶培养物结构很繁琐,并且难以定量分析细胞反应。在当前的研究中,已经开发出用于进行3D细胞培养和分析细胞生存力的新型平台,以实现高通量药物筛选。将封装在水凝胶中的细胞在纸质底物的微孔中培养。然后将底物浸入含有药物的培养基中2天。在文化课程的第24和48小时,将纸基底放置在用于进行阻抗测量的测量电极上,以便定量水凝胶中的细胞生存力。在两种药物(阿霉素和依托泊苷)的治疗下,定量研究了两种人类肝癌细胞系(Huh7和Hep-G2)的细胞活力。以IC表示的结果50个值显示,Huh7细胞比Hep-G2细胞具有更高的耐药性,阿霉素比依托泊苷具有更高的治疗肝细胞癌的功效。当前的工作展示了一种高通量,方便且定量的平台,可用于研究在3D环境中培养的细胞的化学敏感性。

更新日期:2017-09-19
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