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Hydrogelation of a Naphthalene Diimide Appended Peptide Amphiphile and Its Application in Cell Imaging and Intracellular pH Sensing
Biomacromolecules ( IF 6.2 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acs.biomac.7b01048 Nilotpal Singha 1 , Purnima Gupta 2 , Bapan Pramanik 1 , Sahnawaz Ahmed 1 , Antara Dasgupta 1 , Anindita Ukil 2 , Debapratim Das 1
Biomacromolecules ( IF 6.2 ) Pub Date : 2017-09-19 00:00:00 , DOI: 10.1021/acs.biomac.7b01048 Nilotpal Singha 1 , Purnima Gupta 2 , Bapan Pramanik 1 , Sahnawaz Ahmed 1 , Antara Dasgupta 1 , Anindita Ukil 2 , Debapratim Das 1
Affiliation
This study reports the self-assembly and application of a naphthalene diimide (NDI)-appended peptide amphiphile (PA). H-bonding among the peptide moiety in conjunction with π-stacking between NDI and hydrophobic interactions within the alkyl chain are the major driving forces behind the stepwise aggregation of the PA to form hydrogels. The PA produced efficient self-assemblies in water, forming a nanofibrous network that further formed a self-supportive hydrogel. The molecule followed a three-step self-assembly mechanism. At a lower concentration (50 μM), it forms extremely small aggregates with a very low population of the molecules. With an increase in concentration, spherical aggregates are formed above 450 μM concentration. Importantly, this water-soluble conjugate was found to be nontoxic, cell permeable, and usable for cell imaging. Moreover, the aggregation process and consequently the emission behavior are highly responsive to the pH of the medium. Thus, the pH responsive aggregation and emission behavior has an extended biological application for assessing intracellular pH. The biocompatibility and intracellular pH determining capability suggest it is a promising candidate for use as a supramolecular material in biomedical applications.
中文翻译:
萘二酰亚胺附加肽两亲物的水凝胶化及其在细胞成像和细胞内pH传感中的应用
这项研究报告了萘二酰亚胺(NDI)附加的肽两亲物(PA)的自组装和应用。肽部分之间的H键结合NDI和烷基链内的疏水相互作用之间的π堆积是PA逐步聚集形成水凝胶的主要驱动力。PA在水中产生有效的自组装,形成纳米纤维网络,进一步形成自支撑水凝胶。该分子遵循三步自组装机制。在较低的浓度(50μM)下,它会形成非常小的聚集体,并且分子的数量非常少。随着浓度的增加,在浓度高于450μM时会形成球形聚集体。重要的是,发现该水溶性缀合物是无毒的,细胞可渗透的并且可用于细胞成像。而且,聚集过程以及因此的发射行为对介质的pH高度敏感。因此,pH响应的聚集和发射行为在评估细胞内pH值方面具有广泛的生物学应用。生物相容性和细胞内pH值确定能力表明,它是在生物医学应用中用作超分子材料的有希望的候选者。
更新日期:2017-09-19
中文翻译:
萘二酰亚胺附加肽两亲物的水凝胶化及其在细胞成像和细胞内pH传感中的应用
这项研究报告了萘二酰亚胺(NDI)附加的肽两亲物(PA)的自组装和应用。肽部分之间的H键结合NDI和烷基链内的疏水相互作用之间的π堆积是PA逐步聚集形成水凝胶的主要驱动力。PA在水中产生有效的自组装,形成纳米纤维网络,进一步形成自支撑水凝胶。该分子遵循三步自组装机制。在较低的浓度(50μM)下,它会形成非常小的聚集体,并且分子的数量非常少。随着浓度的增加,在浓度高于450μM时会形成球形聚集体。重要的是,发现该水溶性缀合物是无毒的,细胞可渗透的并且可用于细胞成像。而且,聚集过程以及因此的发射行为对介质的pH高度敏感。因此,pH响应的聚集和发射行为在评估细胞内pH值方面具有广泛的生物学应用。生物相容性和细胞内pH值确定能力表明,它是在生物医学应用中用作超分子材料的有希望的候选者。