A new fibroin/dodecanol floating solidification microextraction, coupled with high performance liquid chromatography, was developed and applied for enrichment and quantification of the trace flavonoids in traditional Chinese medicine and biological samples. Also, fibroin sensibilization mechanism was described, and influence of sample matrix to enrichment factor was investigated. In this method, a homogeneous fibroin/dodecanol of dispersed solution was employed as microextraction phase to flavonoids (myricetin, quercetin, isorhamnetin, chrysin, kaempferide), the several critical parameters affecting the performance, such as organic extractant, amount of fibroin in organic extractant, volume of extraction phase, dispersant, salt concentration, pH of sample phase, stirring rate, extraction time, and volume of sample phase were tested and optimized. Under the optimized conditions, enrichment factor of flavonoids ranged from 42.4 to 238.1 in different samples, excellent linearities with r²≥ 0.9968 for all analytes were achieved, limits of detection were less than or equal to 5.0 ng/mL, average recoveries were 92.5% to 115.0% in different samples. The new procedure is simple, fast, low cost, environmentally friendly and high EF, it can also be applied to the concentration and enrichment of the trace flavonoids in other complex matrixes.

开发了一种新的纤维蛋白/十二烷醇悬浮凝固微萃取技术，并结合高效液相色谱法，用于中药和生物样品中痕量类黄酮的富集和定量分析。此外，描述了丝蛋白增敏的机理，并研究了样品基质对富集因子的影响。在该方法中，将分散溶液的均质纤维蛋白/十二烷醇用作黄酮类化合物（杨梅素，槲皮素，异鼠李素，菊花素，山ka素）的微萃取相，影响性能的几个关键参数，例如有机萃取剂，有机萃取剂中的纤维蛋白含量对萃取相的体积，分散剂，盐浓度，样品相的pH，搅拌速率，萃取时间和样品相的体积进行了测试和优化。² ≥0.9968对于所有分析物来实现，检测限小于或等于5.0毫微克/毫升，平均回收率为92.5％至115.0％不同样品英寸 该新方法操作简单，快速，成本低廉，环境友好，EF较高，还可以用于其他复杂基质中痕量类黄酮的浓缩和富集。