This work describes the development and validation of a quantitative and confirmatory method to determination of nine fluoroquinolones residues in poultry, bovine, swine and fish muscle using LC–MS/MS. Sample preparation was based in a fast solvent extraction with acetonitrile with 1% of formic acid followed by a low-temperature clean up procedure and centrifugation, without further steps. The recoveries ranged between 79% and 115%. The concentration work range was 0–200 μg kg⁻¹. The LOD and LOQ were 5 and 10 μg kg⁻¹, respectively. This high-throughput method was reliable for identification and confirmation of nine relevant compounds in food-producing animal tissues. Validation procedure was performed according the Directive 2002/657/EC criteria and the method showed fitness to purpose in terms of precision and reproducibility as well as for the other performance parameters.

这项工作描述了使用LC-MS / MS测定家禽，牛，猪和鱼肉中9种氟喹诺酮类残留物的定量和确认方法的开发和验证。样品制备基于乙腈和1％甲酸的快速溶剂萃取，然后进行低温净化程序和离心分离，无需其他步骤。回收率介于79％和115％之间。浓度工作范围是0–200μgkg ^-1。LOD和LOQ为5和10μgkg ^-1， 分别。这种高通量方法对于食品生产动物组织中的九种相关化合物的鉴定和确证是可靠的。验证程序是根据Directive 2002/657 / EC标准执行的，该方法在准确性和可重复性以及其他性能参数方面显示出适合目的。