Affinity capillary electrophoresis (ACE) has been widely applied to evaluate binding constants of various systems. Recently, moment equations were derived based on the moment analysis (MA) theory for describing the influence of reaction kinetics and longitudinal diffusion on the elution peak profiles measured by ACE (MA-ACE). The equations enable one to obtain not only the binding constants but also the reaction rate constants from the migration time and variance of elution peaks. However, it is necessary to consider other factors (e.g., sample injection, detector window, Joule heating, and ramp time of the voltage increase) to improve the accuracy of MA-ACE. The variance of these effects was quantified under typical experimental conditions. Such quantification clarified the process to obtain the rate constants. The best experimental conditions to achieve high accuracy were discussed.

中文翻译：

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基于亲和毛细管电泳的迁移时间和方差评估，以进行准确的动力学研究

亲和毛细管电泳（ACE）已被广泛用于评估各种系统的结合常数。最近，基于矩量分析（MA）理论推导了矩量方程，用于描述反应动力学和纵向扩散对ACE（MA-ACE）测得的洗脱峰分布的影响。这些方程式不仅使人们能够从迁移时间和洗脱峰的方差中获得结合常数，而且能够获得反应速率常数。但是，有必要考虑其他因素（例如，样品注入，检测器窗口，焦耳热和电压升高的斜坡时间）以提高MA-ACE的准确性。在典型的实验条件下，对这些影响的差异进行了量化。这样的量化澄清了获得速率常数的过程。