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Development of chitosan-sodium phytate nanoparticles as a potent antibacterial agent
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2017-09-17 , DOI: 10.1016/j.carbpol.2017.09.053
Jie Yang , Hao Lu , Man Li , Jing Liu , Shuangling Zhang , Liu Xiong , Qingjie Sun

Chitosan nanoparticles have attracted considerable attention as a potential carrier for food and pharmaceutical applications. Herein, using natural sodium phytate as a gelation agent, we developed a new type of green and biocompatible chitosan nanoparticles. We discovered that the chitosan-sodium phytate nanoparticles exhibited potent antibacterial activities. The chitosan-sodium phytate nanoparticles prepared from low molecular weight (LMW, 140 ± 7 kDa) and medium molecular weight (MMW, 454 ± 21 kDa) chitosan were spherical. Under optimum conditions—with a ratio of LMW chitosan to sodium phytate of 24:1 and MMW chitosan to sodium phytate of 21:1—the sizes of the LMW and MMW chitosan nanoparticles were 20–80 and 80–100 nm, respectively, as observed by transmission electron microscopy. The formation mechanism of chitosan nanoparticles occurred through both electrostatic interactions and hydrogen bonds. No cytotoxicity for normal liver cells was found in chitosan-sodium phytate nanoparticles measured by methyl thiazolyl tetrazolium assay. Furthermore, the antimicrobial assays indicated that the antimicrobial activity of the LMW chitosan nanoparticles was greater than that of MMW chitosan nanoparticles. The minimum inhibition concentration values and half inhibiting concentration of LMW chitosan-sodium phytate nanoparticles for Escherichia coli were 1.5 and 0.8 mg/mL, respectively. In addition, the antimicrobial activity of chitosan nanoparticles against Gram-negative bacteria was better than that against Gram-positive bacteria. The newly developed chitosan-sodium phytate nanoparticles could be used as a potential antibacterial agent.

中文翻译:

壳聚糖植酸钠纳米颗粒作为强效抗菌剂的开发

壳聚糖纳米颗粒作为食品和药物应用的潜在载体已引起了广泛的关注。在本文中,我们使用天然植酸钠作为胶凝剂,开发了一种新型的绿色且生物相容的壳聚糖纳米颗粒。我们发现,壳聚糖植酸钠纳米粒子表现出强大的抗菌活性。由低分子量(LMW,140±7 kDa)和中等分子量(MMW,454±21 kDa)的壳聚糖制备的壳聚糖植酸钠纳米颗粒为球形。在最佳条件下(LMW壳聚糖与植酸钠之比为24:1,MMW壳聚糖与植酸钠之比为21:1),LMW和MMW壳聚糖纳米粒子的大小分别为20–80和80–100 nm通过透射电子显微镜观察。壳聚糖纳米粒子的形成机理是通过静电相互作用和氢键发生的。通过甲基噻唑基四唑鎓测定法测量的壳聚糖-植酸钠钠纳米颗粒中未发现对正常肝细胞的细胞毒性。此外,抗微生物试验表明,LMW壳聚糖纳米颗粒的抗微生物活性大于MMW壳聚糖纳米颗粒的抗微生物活性。LMW壳聚糖植酸钠纳米粒子的最小抑菌浓度值和一半抑菌浓度。抗菌试验表明,LMW壳聚糖纳米颗粒的抗菌活性大于MMW壳聚糖纳米颗粒的抗菌活性。LMW壳聚糖植酸钠纳米粒子的最小抑菌浓度值和一半抑菌浓度。抗菌试验表明,LMW壳聚糖纳米颗粒的抗菌活性大于MMW壳聚糖纳米颗粒的抗菌活性。LMW壳聚糖植酸钠纳米粒子的最小抑菌浓度值和一半抑菌浓度。大肠杆菌分别为1.5和0.8 mg / mL。另外,壳聚糖纳米颗粒对革兰氏阴性菌的抗菌活性优于对革兰氏阳性菌的抗菌活性。新开发的壳聚糖植酸钠纳米颗粒可用作潜在的抗菌剂。
更新日期:2017-09-19
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