Previous studies suggested that expression of most yeast mRNAs is dominated by either transcription factor TFIID or SAGA. We re-examined the role of TFIID by rapid depletion of S. cerevisiae TFIID subunits and measurement of changes in nascent transcription. We find that transcription of nearly all mRNAs is strongly dependent on TFIID function. Degron-dependent depletion of Taf1, Taf2, Taf7, Taf11, and Taf13 showed similar transcription decreases for genes in the Taf1-depleted, Taf1-enriched, TATA-containing, and TATA-less gene classes. The magnitude of TFIID dependence varies with growth conditions, although this variation is similar genome-wide. Many studies have suggested differences in gene-regulatory mechanisms between TATA and TATA-less genes, and these differences have been attributed in part to differential dependence on SAGA or TFIID. Our work indicates that TFIID participates in expression of nearly all yeast mRNAs and that differences in regulation between these two gene categories is due to other properties.

以前的研究表明，大多数酵母 mRNA 的表达由转录因子 TFIID 或 SAGA 主导。我们通过快速消耗酿酒酵母重新检查了 TFIID 的作用TFIID 亚基和新生转录变化的测量。我们发现几乎所有 mRNA 的转录都强烈依赖于 TFIID 功能。Taf1、Taf2、Taf7、Taf11 和 Taf13 的 Degron 依赖性耗尽显示了 Taf1 耗尽、Taf1 富集、TATA 包含和 TATA 少基因类别中基因的类似转录减少。TFIID 依赖性的大小随生长条件而变化，尽管这种变化在全基因组范围内是相似的。许多研究表明 TATA 和 TATA-less 基因之间的基因调控机制存在差异，这些差异部分归因于对 SAGA 或 TFIID 的不同依赖。我们的工作表明，TFIID 参与了几乎所有酵母 mRNA 的表达，这两个基因类别之间的调控差异是由于其他特性。