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Enhanced green fluorescent protein in optofluidic Fabry-Perot microcavity to detect laser induced temperature changes in a bacterial culture
Applied Physics Letters ( IF 4 ) Pub Date : 2017-09-11 , DOI: 10.1063/1.4990870
F. Lahoz 1, 2 , I. R. Martín 1, 3 , D. Walo 1 , R. Freire 4 , J. Gil-Rostra 5 , F. Yubero 5 , A. R. Gonzalez-Elipe 5
Affiliation  

Thermal therapy using laser sources can be used in combination with other cancer therapies to eliminate tumors. However, high precision temperature control is required to avoid damage in healthy surrounding tissues. Therefore, in order to detect laser induced temperature changes, we have used the fluorescence signal of the enhanced Green Fluorescent Protein (eGFP) over-expressed in an E. coli bacterial culture. For that purpose, the bacteria expressing eGFP are injected in a Fabry-Perot (FP) optofluidic planar microcavity. In order to locally heat the bacterial culture, external infrared or ultraviolet lasers were used. Shifts in the wavelengths of the resonant FP modes are used to determine the temperature increase as a function of the heating laser pump power. Laser induced local temperature increments up to 6–7 °C were measured. These results show a relatively easy way to measure laser induced local temperature changes using a FP microcavity and using eGFP as a molecular probe instead of external nanop...

中文翻译:

光流体法布里-珀罗微腔中增强的绿色荧光蛋白可检测细菌培养物中激光诱导的温度变化

使用激光源的热疗法可与其他癌症疗法结合使用以消除肿瘤。然而,需要高精度的温度控制以避免对周围健康组织的损害。因此,为了检测激光诱导的温度变化,我们使用了在大肠杆菌细菌培养物中过度表达的增强型绿色荧光蛋白 (eGFP) 的荧光信号。为此,将表达 eGFP 的细菌注射到法布里-珀罗 (FP) 光流体平面微腔中。为了局部加热细菌培养物,使用了外部红外线或紫外线激光。谐振 FP 模式的波长变化用于确定作为加热激光泵浦功率函数的温度升高。测量了激光引起的局部温度增量高达 6-7 °C。
更新日期:2017-09-11
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