A plethora of publications has continuously reported electrochemical biosensors for detection of pesticides. However, those devices rarely accomplish commercial application due to technical issues associated with the lack of stability and high cost of the biological recognition element (enzyme). Alternatively, the biomimetic catalysts have arisen as a candidate for application in electrochemical biosensors to overcome the enzymatic drawbacks, combining low cost scalable materials with superior stability. Herein, for the first time, we propose a biomimetic biosensor for organophosphorus pesticide detection employing a functionalized polyacrylamide, polyhydroxamicalkanoate (PHA), which mimics the performance of the acetylcholinesterase (AChE) enzyme. The PHA bears functional groups inserted along its backbone chain working as active sites. Thereby, PHA was immobilized on screen printed electrodes (SPE) through a blend formation with poly(ethylene glycol) methyl ether (mPEG) to prevent its leaching out from the surface. Under optimum conditions, the biomimetic sensor was employed for the amperometric detection of paraoxon-ethyl, fenitrothion and chlorpyrifos ranging from 1.0 and 10.0 μmol L⁻¹ with a limit of detection of 0.36 μmol L⁻¹, 0.61 μmol L⁻¹, and 0.83 μmol L⁻¹, respectively. Typical AChE-based interfering species did not affect the PHA performance, which endorsed its superior behavior. The proposed biomimetic biosensor, denoted as SPE/PHA/mPEG, represents a significant advance in the field, offering a new path for low cost devices by means of an artificial enzyme, simple configuration and superior stability. Moreover, the biosensor performance can be further improved by modifying the electrode surface to enhance electronic transfer rate.

大量出版物连续报道了用于检测农药的电化学生物传感器。然而，由于与生物识别元件（酶）的缺乏稳定性和高成本有关的技术问题，这些设备很少实现商业应用。替代地，仿生催化剂已经出现作为在电化学生物传感器中应用的候选者，以克服酶的缺点，将低成本的可扩展材料与优异的稳定性相结合。在此，我们首次提出了一种仿生生物传感器，用于仿效乙酰乙酰胆碱酯酶（AChE）的功能，该仿生生物传感器采用功能化的聚丙烯酰胺聚异羟肟酸酯（PHA）。PHA带有沿其主链插入的官能团，起着活性位点的作用。从而，通过与聚（乙二醇）甲醚（mPEG）的共混形成将PHA固定在丝网印刷电极（SPE）上，以防止其从表面浸出。在最佳条件下，采用仿生传感器对安培克酮，对硫磷，毒死th和毒死detection的安培检测范围为1.0至10.0μmolL^-1与检测的0.36微摩尔L的极限^-1，0.61微摩尔大号^-1，和0.83微摩尔大号^-1分别。典型的基于AChE的干扰物质并未影响PHA的性能，从而证明了其优异的性能。拟议的仿生生物传感器，表示为SPE / PHA / mPEG，代表了该领域的重大进步，它通过人工酶，简单的配置和出色的稳定性为低成本设备提供了一条新途径。此外，可以通过修饰电极表面以提高电子传递速率来进一步提高生物传感器的性能。