A facile solvothermal method for the synthesis of multifunctional magnetic CuFeMnO₄ nanospheres affinity probe (NSAP) with controllable morphology and size was developed for the first time. The CuFeMnO₄ nanospheres combine the brilliant features of Cu²⁺, Fe³⁺, and Mn²⁺ ions, so their multifunction performances are embodied by strong coordination to carboxyl and amine groups of peptides (Cu²⁺ and Fe³⁺), special affinity to phosphate groups of phosphopeptides (Fe³⁺ and Mn²⁺), and high magnetic responsiveness in a magnetic field. Their potential as an affinity probe was evaluated for highly effective enrichment, rapid magnetic separation of low-abundance peptides (neutral condition), and effective selective capture of phosphopeptides (acid condition) from various complex biosamples. Notably, CuFeMnO₄ NSAP was explored for highly selective capture and isolation of phosphopeptides from A549 cells after exposure to ZnO nanoparticles for different times. Consequently, we put forward a new nanospinel ferrite-based protocol here to analyze and identify the phosphoproteins/phosphopeptides involved in cellular signaling pathways in response to exogenous stimulation.

中文翻译：

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可控制备CuFeMnO ₄纳米球作为新型多功能亲和探针，可有效吸附和选择性富集低丰度肽和磷酸肽

首次开发了一种简便的溶剂热方法，用于合成形态和大小可控的多功能磁性CuFeMnO ₄纳米球亲和探针（NSAP）。CuFeMnO ₄纳米球结合了Cu 2 ⁺，Fe ³⁺和Mn ²⁺离子的鲜明特征，因此它们的多功能性能体现在与肽（Cu ²⁺和Fe ³⁺）的羧基和胺基的强配位上。对磷酸肽（Fe ³⁺和Mn ²⁺），以及磁场中的高磁响应性。对它们作为亲和探针的潜力进行了评估，以实现高效富集，低丰度肽的快速磁分离（中性条件）以及有效地从各种复杂生物样品中选择性捕获磷酸肽（酸性条件）。值得注意的是，探索了CuFeMnO ₄ NSAP用于在不同时间暴露于ZnO纳米颗粒后从A549细胞中高度选择性地捕获和分离磷酸肽。因此，我们在这里提出了一种新的基于铁氧体纳米尖晶石的方案，以分析和鉴定响应于外源性刺激而参与细胞信号传导途径的磷蛋白/磷酸肽。