Developing multifunctional phototheranostics with nanoplatforms offers promising potential for effective eradication of malignant solid tumors. In this study, we develop a multifunctional phototheranostic by combining photothermal therapy (PTT) and photoacoustic therapy (PAT) based on a tumor-targeting nanoagent (DBCO-ZnPc-LP). The nanoagent DBCO-ZnPc-LP was facilely prepared by self-assembling of a single lipophilic near-infrared (NIR) dye zinc(II)-phthalocyanine (ZnPc) with a lipid-poly(ethylene glycol) (LP) and following modified further with dibenzyl cyclootyne (DBCO) for introducing the two-step chemical tumor-targeting strategy based on metabolic glycoengineering and click chemistry. The as-prepared DBCO-ZnPc-LP could not only convert NIR light into heat for effective thermal ablation but also induce a thermal-enhanced ultrasound shockwave boost to trigger substantially localized mechanical damage, achieving synergistic antitumor effect both in vitro and in vivo. Moreover, DBCO-ZnPc-LP can be efficiently delivered into tumor cells and solid tumors after being injected intravenously via the two-step tumor-targeting strategy. By integrating the targeting strategy, photoacoustic imaging, and the synergistic interaction between PTT and PAT, a solid tumor could be accurately positioned and thoroughly eradicated in vivo. Therefore, this multifunctional phototheranostic is believed to play an important role in future oncotherapy by the enhanced synergistic effect of PTT and PAT under the guidance of photoacoustic imaging.

中文翻译：

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体内成像引导的光热/光声协同治疗与生物正交代谢糖工程激活肿瘤靶向纳米粒子。

用纳米平台开发多功能光热疗技术为有效根除恶性实体瘤提供了广阔的前景。在这项研究中，我们通过结合基于肿瘤靶向纳米药物（DBCO-ZnPc-LP）的光热疗法（PTT）和光声疗法（PAT）来开发多功能光热疗法。通过将单个亲脂性近红外（NIR）染料锌（II）-酞菁（ZnPc）与脂质-聚（乙二醇）（LP）自组装，可以轻松制备纳米剂DBCO-ZnPc-LP。与二苄基环辛炔（DBCO）一起基于代谢糖工程和点击化学引入了两步化学肿瘤靶向策略。体外和体内。此外，DBCO-ZnPc-LP通过两步靶向肿瘤的策略静脉内注射后，可以有效地递送到肿瘤细胞和实体瘤中。通过整合靶向策略，光声成像以及PTT和PAT之间的协同相互作用，可以在体内准确定位并彻底根除实体瘤。因此，通过在光声成像的指导下增强PTT和PAT的协同作用，认为这种多功能光热疗在未来的肿瘤治疗中起着重要的作用。