当前位置: X-MOL 学术Anal. Chim. Acta › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Colorimetric analysis of lipopolysaccharides based on its self-assembly to inhibit ion transport
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2017-11-01 , DOI: 10.1016/j.aca.2017.09.002
Defeng Li , Tuanwei Sun , Wenjun Zhang , Zhongming Shen , Juan Zhang

A new strategy is proposed based on inhibition of ion transport by lipid bilayer derived from spontaneous assembly of lipopolysaccharides (LPS), thereby a colorimetric method is established for analysis of LPS. At acidic pH values, LPS can specially bind with aminophenylboronic acid modified assembled magnetic nanospheres (APBA/AMNSs), resulting in formation of lipid bilayer around APBA/AMNSs. Under acidic condition, the lipid bilayer can inhibit the release of iron ions from AMNSs into the solution so as to decrease the oxidized extent of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt mediated by hydrogen peroxide. Using the established method, LPS can be detected over the wide linear detection range with the low detection limit. With good selectivity, reproductivity, and simplicity, the method is accurate in LPS tests of real drinking samples.

中文翻译:

基于脂多糖自组装抑制离子转运的比色分析

提出了一种基于脂多糖(LPS)自发组装的脂质双层抑制离子传输的新策略,从而建立了一种用于分析LPS的比色法。在酸性 pH 值下,LPS 可以与氨基苯基硼酸修饰的组装磁性纳米球 (APBA/AMNSs) 特异性结合,导致在 APBA/AMNSs 周围形成脂质双层。在酸性条件下,脂质双层可以抑制铁离子从AMNSs释放到溶液中,从而降低氢介导的2,2'-叠氮基-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐的氧化程度过氧化物。使用已建立的方法,可以在较宽的线性检测范围内以低检测限检测 LPS。具有良好的选择性、重现性和简单性,
更新日期:2017-11-01
down
wechat
bug