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The early nucleation stage of gold nanoparticles formation in solution as powerful tool for the colorimetric determination of reducing agents: The case of xylitol and total polyols in oral fluid
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2017-11-01 , DOI: 10.1016/j.aca.2017.09.020
S. Scarano , E. Pascale , M. Minunni

Herein we report a simple non-enzymatic assay for xylitol and total polyols in water and oral fluid based on the time resolved formation of gold NPs in solution, and their colorimetric detection at fixed wavelength (520 nm). The key novelty of the proposed approach relies on the exploitation of information given by the early nucleation step of NPs formation instead of those related to final products at the end point of AuNPs growth, as generally reported in literature. We demonstrate that the nucleation stage is linearly correlated to the concentration of the reducing agent in solution. On the contrary, the optical reading carried out the end point of the reaction shows non-linear correlation and several undesired features. As case study, we applied the proposed method to xylitol and polyols determination, first tested in water and spiked oral fluid samples. The detection limits obtained on xylitol resulted 180 mg L-1 (CV% = 6.9) and 44 mg L-1 (CV% = 6.5) in water and oral fluid, respectively. Afterward, we successfully performed the monitoring of total polyols in oral fluid over time during xylitol-containing gums consumption. Data here reported show high correspondence with available data in literature. The proposed approach is fast, cheap, highly reproducible, and can be extended to other reducing substances of interest for analytical purposes.

中文翻译:

金纳米粒子在溶液中形成的早期成核阶段作为还原剂比色测定的有力工具:口腔液中木糖醇和总多元醇的案例

在此,我们报告了一种简单的非酶法检测水和口腔液中的木糖醇和总多元醇,其基于溶液中金纳米颗粒的时间分辨形成,以及它们在固定波长 (520 nm) 下的比色检测。所提出的方法的关键新颖之处在于利用 NPs 形成的早期成核步骤提供的信息,而不是那些与 AuNPs 生长终点的最终产品相关的信息,如文献中普遍报道的那样。我们证明成核阶段与溶液中还原剂的浓度线性相关。相反,在反应终点进行的光学读数显示非线性相关性和一些不希望的特征。作为案例研究,我们将所提出的方法应用于木糖醇和多元醇的测定,首先在水和加标口腔液样品中进行测试。木糖醇的检测限导致水和口腔液中分别为 180 mg L-1 (CV% = 6.9) 和 44 mg L-1 (CV% = 6.5)。之后,我们成功地在含木糖醇的口香糖消费期间随时间推移监测了口腔液中的总多元醇。此处报告的数据显示与文献中的可用数据高度对应。所提出的方法快速、廉价、重现性高,并且可以扩展到其他感兴趣的还原物质以进行分析。此处报告的数据显示与文献中的可用数据高度对应。所提出的方法快速、廉价、重现性高,并且可以扩展到其他感兴趣的还原物质以进行分析。此处报告的数据显示与文献中的可用数据高度对应。所提出的方法快速、廉价、重现性高,并且可以扩展到其他感兴趣的还原物质以进行分析。
更新日期:2017-11-01
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