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Instrumentation and applications of electrochemistry coupled to mass spectrometry for studying xenobiotic metabolism: A review
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2017-11-01 , DOI: 10.1016/j.aca.2017.08.050
Lenka Portychová , Kevin A. Schug

The knowledge of metabolic pathways and biotransformation of xenobiotics, artificial substances foreign to the entire biological system, is crucial for elucidation of degradation routes of potentially toxic substances. Nowadays, there are many methods to simulate xenobiotic metabolism in the human body in vitro. In this review, the metabolism of various substances in the human body is described, followed by a summary of methods used for prediction of metabolic pathways and biotransformation. Above all, focus is placed on the coupling of electrochemistry to mass spectrometry, which is still a relatively new technique. This promising tool can mimic both oxidative phase I and conjugative phase II metabolism. Different experimental arrangements, with or without a separation step, and various applications of this technique are illustrated and critically reviewed.

中文翻译:

电化学与质谱联用在异生物质代谢研究中的仪器和应用:综述

异生物质(整个生物系统外的人造物质)的代谢途径和生物转化的知识对于阐明潜在有毒物质的降解途径至关重要。目前,在体外模拟人体内异生物质代谢的方法有很多。在这篇综述中,描述了各种物质在人体内的代谢,然后总结了用于预测代谢途径和生物转化的方法。最重要的是,重点放在电化学与质谱的耦合上,这仍然是一项相对较新的技术。这种有前途的工具可以模拟氧化相 I 和结合相 II 代谢。不同的实验安排,有或没有分离步骤,
更新日期:2017-11-01
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