An imidazo [1,5-α]pyridine-based fluorescent probe MIPY-DNP with a large Stokes shift (173 nm) for the sensing of thiophenols in aqueous medium has been developed. This probe with 2,4-dinitrophenyl ether as a highly thiophenol-selective group was constructed based on the combination of PET and ESIPT mechanisms. Upon the treatment with thiophenols, MIPY-DNP produced a remarkable fluorescence enhancement (209-fold) at 478 nm. The detect limit for thiophenols was calculated to be as low as 5.6 nM. Importantly, the practical utility of MIPY-DNP for the selective detection of thiophenols has been successfully demonstrated in both real water samples and living cells.

中文翻译：

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基于咪唑并[1,5-α]吡啶在水介质和活细胞中检测苯硫酚的大斯托克斯位移荧光探针

已经开发了一种基于咪唑并 [1,5-α] 吡啶的荧光探针 MIPY-DNP，具有大的斯托克斯位移 (173 nm)，用于检测水性介质中的苯硫酚。这种以 2,4-二硝基苯基醚作为高度苯硫酚选择性基团的探针是基于 PET 和 ESIPT 机制的组合构建的。在用苯硫酚处理后，MIPY-DNP 在 478 nm 处产生了显着的荧光增强（209 倍）。计算得出苯硫酚的检测限低至 5.6 nM。重要的是，MIPY-DNP 在选择性检测苯硫酚方面的实用性已在真实水样和活细胞中得到成功证明。