A high sensitive surface plasmon resonance (SPR) biosensor was proposed based on the gold nanoparticles-decorated molybdenum sulfide (AuNPs-MoS2) for the first time. This SPR platform using the AuNPs-MoS2 nanocomposites as signal labels for the sensitive and facile measurement of microRNA (miRNA) was executed in only two steps. At first, the thiol-modified DNA oligonucleotide probes, including a sequence complementary to the target miRNA-141, were fixed on the Au film to identify the segment sequence of target miRNA-141. Then, the assistant DNA-linked AuNPs-MoS2 nanocomposites were used to combine with the other section of the miRNA-141. Performance of SPR biosensor was enhanced by taking advantage of the AuNPs-MoS2 nanocomposites, thus this newly presented sensing assay exhibited high sensitivity toward miRNA with a detection limit of 0.5 fM. Furthermore, the method showed high specificity, resulting in distinguishing differences among miRNA-200 family members. Especially, the assay could also be used to detect human miRNA from cancer cells, and the results were in excellent accord with the ones obtained using qRT-PCR. What is more, this presented method could be feasible for determining miRNA in 10% human serum. This assay may provide a great potential as a miRNA quantification method in complex samples, and exert significant effect on biomedical research and clinical early diagnosis.

中文翻译：

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基于金纳米粒子修饰的硫化钼的高灵敏度表面等离子体共振生物传感器检测微小RNA

首次提出了一种基于金纳米粒子修饰的硫化钼（AuNPs-MoS2）的高灵敏度表面等离子体共振（SPR）生物传感器。该 SPR 平台使用 AuNPs-MoS2 纳米复合材料作为信号标记，用于灵敏、简便地测量 microRNA (miRNA)，仅需两步即可执行。首先，将巯基修饰的 DNA 寡核苷酸探针，包括与目标 miRNA-141 互补的序列，固定在 Au 膜上，以识别目标 miRNA-141 的片段序列。然后，使用辅助 DNA 连接的 AuNPs-MoS2 纳米复合材料与 miRNA-141 的另一部分结合。通过利用 AuNPs-MoS2 纳米复合材料增强了 SPR 生物传感器的性能，因此这种新提出的传感检测对 miRNA 表现出高灵敏度，检测限为 0.5 fM。此外，该方法显示出高特异性，可区分 miRNA-200 家族成员之间的差异。特别是，该方法还可用于检测癌细胞中的人类 miRNA，其结果与使用 qRT-PCR 获得的结果非常吻合。更重要的是，这种提出的方​​法对于确定 10% 人血清中的 miRNA 是可行的。该方法作为复杂样本中miRNA定量方法具有巨大潜力，对生物医学研究和临床早期诊断具有重要意义。该方法可用于测定 10% 人血清中的 miRNA。该方法作为复杂样本中miRNA定量方法具有巨大潜力，对生物医学研究和临床早期诊断具有重要意义。该方法可用于测定 10% 人血清中的 miRNA。该方法作为复杂样本中miRNA的定量方法具有巨大的潜力，对生物医学研究和临床早期诊断具有重要意义。