Biohybrid chitosan-silica scaffolds were synthesized through the sol-gel and the freeze drying processes. Hydrolysis and condensation of chitosan with tetraethylorthosilicate (TEOS) in the presence of 3-isocyanatopropyl triethoxysilane (ICPTES) were successfully carried out. Results obtained from FTIR, swelling test and pyrolysis confirmed that the hybrid scaffolds containing covalent coupling between the organic and inorganic networks were formed with high crosslink density of Si-O-Si bridging and could be classified as the class II material. The hybridization also resulted in improvements on mechanical strength and stability comparing to the pure chitosan. In vitro investigations on the guided bone regeneration and the cytotoxicity were also performed. SEM-EDS was used to examine the proliferation of calcium phosphate mineral at the scaffold surface after an immersion in simulated body fluid. The results revealed that the hybrid scaffolds exhibited a rapid induction of calcium phosphate mineral without cytotoxicity effect, reflecting an excellent in vitro bone bioactivity which was superior to the pure chitosan scaffold.

中文翻译：

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壳聚糖-二氧化硅的无细胞毒性杂化生物支架：溶胶-凝胶的合成，表征及拟议的应用

通过溶胶-凝胶法和冷冻干燥法合成了生物杂合壳聚糖-二氧化硅支架。在3-异氰酸根合丙基三乙氧基硅烷（ICPTES）的存在下，壳聚糖与原硅酸四乙酯（TEOS）的水解和缩合反应得以成功进行。从FTIR，溶胀试验和热解获得的结果证实，在有机和无机网络之间包含共价偶联的杂化支架形成了具有高交联密度的Si-O-Si桥联，可以归为II类材料。与纯壳聚糖相比，杂交还提高了机械强度和稳定性。体外还对引导骨的再生和细胞毒性进行了研究。SEM-EDS用于检查浸泡在模拟体液中后支架表面磷酸钙矿物质的增殖。结果表明，杂种支架表现出对磷酸钙矿物质的快速诱导而没有细胞毒性作用，反映出优异的体外骨生物活性，其优于纯壳聚糖支架。