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From 2D to 3D: the morphology, proliferation and differentiation of MC3T3-E1 on silk fibroin/chitosan matrices
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2017-09-12 , DOI: 10.1016/j.carbpol.2017.09.035
Da-Wei Li , Feng-Li He , Jin He , Xudong Deng , Ya-Li Liu , Yang-Yang Liu , Ya-Jing Ye , Da-Chuan Yin

It has been widely accepted that cell culture in two-dimensional (2D) conditions may not be able to represent growth in three-dimensional (3D) conditions. Systematic comparisons between 2D and 3D cell cultures are needed to appropriately use the existing 2D results. In this work, we conducted a comparative study between 2D and 3D cell cultures of MC3T3-E1 using the same type of material (a mixture of silk fibroin (SF) and chitosan (CS)). Our results showed 3D SF/CS scaffold exhibited different effects on cell culture compared with the 2D cases. 1) The cells grown in 3D scaffold showed multiple morphologies. 2) The proliferation of cells in 3D scaffold was long-term and sustainable. 3) Cell differentiation occurred throughout the entire 3D scaffold. The results showed that cell culture in 3D SF/CS scaffold exhibited different features than 2D cases and 3D SF/CS scaffold could be a promising material for 3D cell culture.

中文翻译:

从2D到3D:丝素蛋白/壳聚糖基质上MC3T3-E1的形态,增殖和分化

二维(2D)条件下的细胞培养可能无法代表三维(3D)条件下的生长,这一点已被广泛接受。为了适当地使用现有的2D结果,需要在2D和3D细胞培养之间进行系统比较。在这项工作中,我们使用相同类型的材料(丝素蛋白(SF)和壳聚糖(CS)的混合物)对MC3T3-E1的2D和3D细胞培养进行了比较研究。我们的结果表明,与2D病例相比,3D SF / CS支架对细胞培养表现出不同的作用。1)在3D支架中生长的细胞显示出多种形态。2)3D支架中的细胞增殖是长期且可持续的。3)在整个3D支架中都发生了细胞分化。
更新日期:2017-09-13
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