Xylo-oligosaccharides and xylo-polysaccharides (XOS, XPS) produced by autohydrolysis of the fibre from oil palm empty fruit bunches (OPEFB) were purified using gel filtration chromatography to separate the XOS and XPS from the crude autohydrolysis liquor. Six mixed fractions of refined XOS and XPS with average degree of polymerisation (avDP) of 4-64 were obtained. These were characterised in terms of their composition and size by HPLC, MALDI-ToF-MS (selected fractions) and carbohydrate gel electrophoresis (PACE). They were assessed in batch culture fermentations using faecal inocula to determine their ability to modulate the human faecal microbiota in vitro by measuring the bacterial growth, organic acid production and the XOS assimilation profile. The gut microbiota was able to utilise all the substrates and there was a link between the avDP with the fermentation properties. In general, XOS/XPS preparations of lower avDP promote better Bifidobacterium growth and organic acid production.

中文翻译：

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人粪便细菌对不同分子量木糖寡糖和木糖多糖组分的体外发酵能力

使用凝胶过滤色谱法纯化从油棕空果束（OPEFB）中纤维自动水解产生的木糖寡糖和木糖多糖（XOS，XPS），从粗制自水解液中分离XOS和XPS。获得了平均聚合度（avDP）为4-64的精制XOS和XPS的六个混合馏分。通过HPLC，MALDI-ToF-MS（选择的馏分）和碳水化合物凝胶电泳（PACE）对它们的组成和大小进行了表征。在使用粪便接种物的分批培养发酵中对它们进行了评估，以确定它们在体外调节人类粪便微生物群的能力。通过测量细菌的生长，有机酸的产生和XOS的吸收曲线。肠道菌群能够利用所有底物，并且avDP与发酵特性之间存在联系。通常，较低avDP的XOS / XPS制剂可促进更好的双歧杆菌生长和有机酸生成。