Chikungunya virus (CHIKV) infection leads to activation of innate immune response by triggering Toll-like receptor (TLR) pathways resulting in elevated cytokines and type-I interferon levels. Genetic variations of these genes may influence human CHIKV-susceptibility and disease progression. Present study aimed to identify role of TLR polymorphisms in CHIKV-susceptibility and their association with cytokines and clinical symptoms. This is the first study illustrating certain genotypes of TLR-7 and TLR-8 SNPs viz. CT(p = 0.002)]; rs3853839[GC(p < 0.001), CC(p = 0.039)] and rs3764879[GC(p < 0.001)] were considerably associated with CHIKV susceptibility. Increased risk of CHIKV infection among male patients with CC-genotype (rs179010) (p = 0.028) and female patients with GT-genotype (rs5741880) (p = 0.019) was observed. Significant higher IFN-α (P = 0.002) levels among chikungunya TNF-α (P = 0.034) patients was reported. Chikungunya patients with rs179010-CC genotype showed significantly high IFN-α level(p = 0.003). Thus, these TLR variants might act as potential prognostic biomarkers for predicting CHIKV susceptibility among uninfected individuals.

基孔肯雅病毒（CHIKV）感染通过触发Toll样受体（TLR）途径导致先天免疫应答激活，从而导致细胞因子和I型干扰素水平升高。这些基因的遗传变异可能会影响人类对CHIKV的敏感性和疾病进展。本研究旨在确定TLR基因多态性在CHIKV易感性中的作用及其与细胞因子和临床症状的关系。这是第一项说明某些TLR-7和TLR-8 SNP基因型的研究。CT（p = 0.002）]；rs3853839 [GC（p <0.001），CC（p = 0.039）]和rs3764879 [GC（p <0.001）]与CHIKV易感性密切相关。观察到CC基因型男性患者（rs179010）（p = 0.028）和GT基因型女性患者（rs5741880）（p = 0.019）的CHIKV感染风险增加。据报道，基孔肯雅病TNF-α（P = 0.034）患者中IFN-α水平显着升高（P = 0.002）。具有rs179010-CC基因型的基孔肯雅病患者表现出明显较高的IFN-α水平（p = 0.003）。因此，这些TLR变异体可作为预测未感染个体中CHIKV易感性的潜在预后生物标志物。