当前位置: X-MOL 学术Virology › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Validation of reference genes for quantifying changes in gene expression in virus-infected tobacco
Virology ( IF 3.7 ) Pub Date : 2017-07-07 , DOI: 10.1016/j.virol.2017.06.029
Eseul Baek , Ju-Yeon Yoon , Peter Palukaitis

To facilitate quantification of gene expression changes in virus-infected tobacco plants, eight housekeeping genes were evaluated for their stability of expression during infection by one of three systemically-infecting viruses (cucumber mosaic virus, potato virus X, potato virus Y) or a hypersensitive-response-inducing virus (tobacco mosaic virus; TMV) limited to the inoculated leaf. Five reference-gene validation programs were used to establish the order of the most stable genes for the systemically-infecting viruses as ribosomal protein L25 > β-Tubulin > Actin, and the least stable genes Ubiquitin-conjugating enzyme (UCE) < PP2A < GAPDH. For local infection by TMV, the most stable genes were EF1α > Cysteine protease > Actin, and the least stable genes were GAPDH < PP2A < UCE. Using two of the most stable and the two least stable validated reference genes, three defense responsive genes were examined to compare their relative changes in gene expression caused by each virus.



中文翻译:

验证参考基因以定量感染病毒的烟草中基因表达的变化

为了便于量化受病毒感染的烟草植物中基因表达变化的数量,评估了三种看家基因在三种系统感染病毒(黄瓜花叶病毒,马铃薯X病毒,马铃薯Y病毒)之一或超敏病毒感染过程中的表达稳定性。应答病毒(烟草花叶病毒; TMV)仅限于接种的叶片。使用五个参考基因验证程序来确定全身感染病毒的最稳定基因的顺序,如核糖体蛋白L25>β-微管蛋白>肌动蛋白,最不稳定的基因泛素结合酶(UCE)<PP2A <GAPDH 。对于TMV局部感染,最稳定的基因是EF1α>半胱氨酸蛋白酶>肌动蛋白,最不稳定的基因是GAPDH <PP2A <UCE。

更新日期:2017-07-07
down
wechat
bug