In the present study, a novel enzymatic glucose biosensor using glucose oxidase (GOx) immobilized into (3-aminopropyl) triethoxysilane (APTES) functionalized reduced graphene oxide (rGO-APTES) and hydrogen peroxide sensor based on rGO-APTES modified glassy carbon (GC) electrode were fabricated. Nafion (Nf) was used as a protective membrane. For the characterization of the composites, Fourier transform infrared spectroscopy (FTIR), X-ray powder diffractometer (XRD), and transmission electron microscopy (TEM) were used. The electrochemical properties of the modified electrodes were investigated using electrochemical impedance spectroscopy, cyclic voltammetry, and amperometry. The resulting Nf/rGO-APTES/GOx/GC and Nf/rGO-APTES/GC composites showed good electrocatalytical activity toward glucose and H2O2, respectively. The Nf/rGO-APTES/GC electrode exhibited a linear range of H2O2 concentration from 0.05 to 15.25 mM with a detection limit (LOD) of 0.017 mM and sensitivity of 124.87 μA mM−1 cm−2. The Nf/rGO-APTES/GOx/GC electrode showed a linear range of glucose from 0.02 to 4.340 mM with a LOD of 9 μM and sensitivity of 75.26 μA mM−1 cm−2. Also, the sensor and biosensor had notable selectivity, repeatability, reproducibility, and storage stability.

中文翻译：

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基于（3-氨基丙基）三乙氧基硅烷官能化还原氧化石墨烯的新型酶促葡萄糖生物传感器和非酶促过氧化氢传感器

在本研究中，一种使用固定在（3-氨基丙基）三乙氧基硅烷（APTES）功能化还原氧化石墨烯（rGO-APTES）中的葡萄糖氧化酶（GOx）和基于 rGO-APTES 改性玻碳（GC）的过氧化氢传感器的新型酶促葡萄糖生物传感器) 电极。Nafion (Nf) 用作保护膜。对于复合材料的表征，使用了傅里叶变换红外光谱 (FTIR)、X 射线粉末衍射仪 (XRD) 和透射电子显微镜 (TEM)。使用电化学阻抗谱、循环伏安法和电流法研究了修饰电极的电化学性质。所得的 Nf/rGO-APTES/GOx/GC 和 Nf/rGO-APTES/GC 复合材料分别对葡萄糖和 H2O2 显示出良好的电催化活性。Nf/rGO-APTES/GC 电极的 H2O2 浓度线性范围为 0.05 至 15.25 mM，检测限 (LOD) 为 0.017 mM，灵敏度为 124.87 μA mM-1 cm-2。Nf/rGO-APTES/GOx/GC 电极显示葡萄糖的线性范围为 0.02 至 4.340 mM，LOD 为 9 μM，灵敏度为 75.26 μA mM-1 cm-2。此外，传感器和生物传感器具有显着的选择性、重复性、再现性和储存稳定性。