AbstractMagnetic molecularly imprinted nanoparticles (MMIPs) with improved dispersity and an increased number of adsorption sites are described. Uniform silica layers were first deposited on the surface of Fe3O4 nanoparticles (Fe3O4 NPs) in order to improve the dispersity of magnetic nanoparticles. Then, 4-formylphenylboronic acid (FPBA) as functional monomer was immobilized on the magnetic carriers to improve the efficiency of template eluting and rebinding. A thin layer of polyaniline imprinted with horseradish peroxidase (HRP) as a model glycoprotein was then placed on the magnetic nanoparticles to enhance the dispersity of the resultant MMIPs. These exhibit high adsorption capacity (62 mg g−1), a satisfactory imprinting factor ( 3.78) and short adsorption equilibrium time (40 min) toward HRP, and the limit of detection is 18.7 μg L−1. This kind of MMIPs, therefore, is deemed being a useful tool for extracting low-abundance glycoproteins from even complex samples. Graphical abstractSchematic of the preparation of magnetic molecular imprinted nanoparticles using Fe3O4 nanoparticles as carriers, 4-formylphenylboronic acid as functional monomer, aniline as cross linker and horseradish peroxidase as template. TEOS: tetraethyl orthosilicate; APTES: 3-aminopropyltriethoxysilane; FPBA: 4-formylphenylboronic acid; HRP: horseradish peroxidase.

中文翻译：

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基于硼酸盐亲和性的磁性分子印迹纳米粒子，用于有效提取模型糖蛋白辣根过氧化物酶

摘要描述了具有改善的分散性和增加的吸附位点数量的磁性分子印迹纳米粒子 (MMIP)。首先在 Fe3O4 纳米粒子 (Fe3O4 NPs) 表面沉积均匀的二氧化硅层，以提高磁性纳米粒子的分散性。然后，将4-甲酰基苯基硼酸（FPBA）作为功能单体固定在磁性载体上，以提高模板洗脱和重新结合的效率。然后将印有辣根过氧化物酶 (HRP) 作为模型糖蛋白的聚苯胺薄层置于磁性纳米颗粒上，以增强所得 MMIP 的分散性。这些表现出高吸附容量 (62 mg g-1)、令人满意的印迹因子 (3.78) 和对 HRP 的短吸附平衡时间 (40 分钟)，检测限为 18.7 μg L-1。因此，这种 MMIPs 被认为是从复杂样品中提取低丰度糖蛋白的有用工具。图解摘要以Fe3O4纳米颗粒为载体，4-甲酰基苯基硼酸为功能单体，苯胺为交联剂，辣根过氧化物酶为模板制备磁性分子印迹纳米颗粒的示意图。TEOS：原硅酸四乙酯；APTES：3-氨基丙基三乙氧基硅烷；FPBA：4-甲酰基苯基硼酸；HRP：辣根过氧化物酶。苯胺作为交联剂，辣根过氧化物酶作为模板。TEOS：原硅酸四乙酯；APTES：3-氨基丙基三乙氧基硅烷；FPBA：4-甲酰基苯基硼酸；HRP：辣根过氧化物酶。苯胺作为交联剂，辣根过氧化物酶作为模板。TEOS：原硅酸四乙酯；APTES：3-氨基丙基三乙氧基硅烷；FPBA：4-甲酰基苯基硼酸；HRP：辣根过氧化物酶。