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Quantitative evaluation and in vivo visualization of mercury ion bioaccumulation in rotifers by novel aggregation-induced emission fluorogen nanoparticles
Environmental Science: Nano ( IF 7.3 ) Pub Date : 2017-09-12 00:00:00 , DOI: 10.1039/c7en00599g
Yusheng Jiang 1, 2, 3, 4, 5 , Tao He 4, 5, 6, 7, 8 , Yuncong Chen 3, 9, 10 , Yinlan Ruan 6, 11, 12, 13 , Yabin Zhou 5, 6, 14 , Ben Zhong Tang 3, 9, 10 , Jianguang Qin 4, 5, 6 , Youhong Tang 5, 6, 14
Affiliation  

In this study, the specifically-designed aggregation-induced emission fluorogen (AIEgen) with nanoparticle aggregates was used to quantitatively evaluate the bioaccumulation of Hg2+ and in vivo visualize Hg2+ kinetics within the rotifer Brachionus plicatilis for the first time. Quantitative results showed that a sharp drop in Hg2+ concentration occurred at the very beginning in the medium containing rotifers and Hg2+, showing a quick initial uptake of Hg2+ by rotifers and reaching a plateau concentration in the medium after 5 min. With an increase in rotifer density, the amount of bioaccumulation increased in the rotifer. However, the bioaccumulation efficiency of Hg2+ decreased from 5.28 µg/mg/h at a low rotifer density of 0.093 mg /ml to 2.61 µg/mg/h at a high rotifer density of 0.375 mg/mL. Moreover, the fluorescent images and spectra results illustrated that the ingestion of Hg2+ in the rotifer was via the mouth surrounded by the ciliary corona to the digestive tract, and Hg2+ could not permeate into the body integument through diffusion at the study period. Hg2+-induced fluorescence in rotifers dissipated in 6 h after staining possibly through defecation and excretion. This study indicates that inorganic mercury can be quickly ingested by a rotifer via feeding, but is unlikely deposited as methylated mercury in rotifer tissues.

中文翻译:

新型聚集诱导的发射氟纳米粒子对轮虫中汞离子生物富集的定量评估和体内可视化

在这项研究中,专门设计的具有纳米颗粒聚集体的聚集诱导的发射氟气(AIEgen)用于定量评估Hg2 +的生物蓄积性,并首次在体内观察轮虫Brachionus plicatilis中的Hg2 +动力学。定量结果表明,在含有轮虫和Hg2 +的培养基中,Hg2 +浓度在开始时就出现了急剧下降,这表明轮虫快速吸收了Hg2 +,并在5分钟后达到了平台浓度。随着轮虫密度的增加,轮虫中的生物积累量增加。但是,Hg2 +的生物积累效率从低轮虫密度0.093 mg / ml时的5.28 µg / mg / h降低到高轮虫密度0.375 mg / mL时的2.61 µg / mg / h。而且,荧光图像和光谱结果表明,轮虫中的Hg2 +摄入是通过被睫状冠状体包围的口腔到达消化道,并且在研究期间Hg2 +不会通过扩散渗透到体内的皮被中。Hg2 +诱导的轮虫荧光在染色后6小时内消散,可能是通过排便和排泄。这项研究表明,轮虫可以通过进食快速摄取无机汞,但不太可能以甲基化汞的形式沉积在轮虫组织中。Hg2 +诱导的轮虫荧光在染色后6小时内消散,可能是通过排便和排泄。这项研究表明,轮虫可以通过进食快速摄取无机汞,但不太可能以甲基化汞的形式沉积在轮虫组织中。Hg2 +诱导的轮虫荧光在染色后6小时内消散,可能是通过排便和排泄。这项研究表明,轮虫可以通过进食快速摄取无机汞,但不太可能以甲基化汞的形式沉积在轮虫组织中。
更新日期:2017-09-12
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