To assess the dynamics of microbial communities in a petroleum reservoir during microbial enhanced oil recovery (MEOR), injected and produced fluids from multiple wells were monitored using molecular microbial methods over 20 months. In this highly permeable (1.5–2.5 μm²) and high-temperature (65 °C) reservoir, communities contain phyla Euryarchaeota, Proteobacteria, Deferribacteres, and Firmicutes, which may be collected by flooding fluids from different habitats through strata. Since the oil-rich areas in the flooded reservoir generally gather around oil wells with high temperatures and strictly anaerobic conditions, the dominance of thermophilic and anaerobic microorganisms, which are capable of inhabiting oil-rich areas, is consistent with positive oil-output responses (temporarily enhanced by 5 × 10³ kg per day). During later periods, the communities were dominated by Enterobacter without high-temperature adaptability, which corresponds to a considerable decline in oil-output. Meanwhile, an abnormal increase of community similarity, acetate, and cell concentrations in produced fluids simultaneously indicated a severe enhancement of reservoir permeability along the flooding route, which reveals the direct reason for the community shift and the oil output decline. Therefore, an understanding of the long-term dynamics of reservoir communities is essential for distinguishing functional species and to establish a reservoir-scale connection between microbiology and porous flow.

中文翻译：

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高渗透性油藏中微生物群落的长期动态揭示了群落与采油量之间的时空关系

为了评估微生物强化油采收（MEOR）期间石油储层中微生物群落的动态，使用分子微生物方法在20个月内监测了多口井的注水和采出液。在这种高渗透性（1.5-2.5微米²）和高温（65℃）贮存，社区含有门类广古菌门，变形菌，Deferribacteres，和厚壁菌门，其可以由通过地层驱不同产地的流体被收集。由于淹没油藏中的富油区通常聚集在高温和严格厌氧条件下的油井周围，因此能够居住在富油区的嗜热和厌氧微生物占主导地位，这与积极的石油产出反应是一致的（暂时增强5×10每天³公斤）。在后来的时期，这些社区以肠杆菌为主导，而没有高温适应性，这对应于石油产量的显着下降。同时，社区相似度，乙酸盐和采出液中细胞浓度的异常增加同时表明沿洪水路线的储层渗透率大大增加，这揭示了社区转移和石油产量下降的直接原因。因此，对储层群落长期动态的了解对于区分功能物种和建立微生物学与多孔流之间的储层规模联系至关重要。