Revealing pathogens: A ratiometric fluorescent probe (CTX-HN) for the detection of extended-spectrum β-lactamase (ESBL) activity is reported. This reagent exhibits a change in fluorescent ratio of over 2500-fold upon enzymatic hydrolysis. Adoption of the core structure of cefotaxime as an enzymatic recognition moiety in this probe results in excellent selectivity to ESBLs over other prevalent β-lactamases.

中文翻译：

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用比率荧光探针特异性检测广谱β-内酰胺酶活性

揭示病原体：报告了用于检测超广谱β-内酰胺酶（ESBL）活性的比例荧光探针（CTX-HN）。酶水解后，该试剂的荧光比率变化超过2500倍。在该探针中采用头孢噻肟的核心结构作为酶促识别部分，与其他普遍的β-内酰胺酶相比，对ESBLs具有极好的选择性。