Injectable, dual-responsive, and degradable poly(oligo ethylene glycol methacrylate) (POEGMA) hydrogels are demonstrated to offer potential for cell delivery. Charged groups were incorporated into hydrazide and aldehyde-functionalized thermoresponsive POEGMA gel precursor polymers via the copolymerization of N,N′-dimethylaminoethyl methacrylate (DMAEMA) or acrylic acid (AA) to create dual-temperature/pH-responsive in situ gelling hydrogels that can be injected via narrow gauge needles. The incorporation of charge significantly broadens the swelling, degradation, and rheological profiles achievable with injectable POEGMA hydrogels without significantly increasing nonspecific protein adsorption or chronic inflammatory responses following in vivo subcutaneous injection. However, significantly different cell responses are observed upon charge incorporation, with charged gels significantly improving 3T3 mouse fibroblast cell adhesion in 2D and successfully delivering viable and proliferating ARPE-19 human retinal epithelial cells via an “all-synthetic” matrix that does not require the incorporation of cell-adhesive peptides.

中文翻译：

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具有可调节电荷密度的可注射和降解的聚甲基丙烯酸低聚乙二醇水凝胶作为无肽无细胞支架

已证明可注射，双重反应和可降解的聚（甲基丙烯酸低聚乙二醇酯）（POEGMA）水凝胶可为细胞递送提供潜力。通过N，N的共聚将带电荷的基团掺入酰肼和醛官能化的热响应性POEGMA凝胶前体聚合物中甲基丙烯酸'-二甲基氨基乙基酯（DMAEMA）或丙烯酸（AA）产生双温度/ pH响应原位凝胶化水凝胶，可通过窄口针注射。电荷的掺入显着拓宽了可注射的POEGMA水凝胶所能达到的溶胀，降解和流变特性，而在体内皮下注射后并未显着增加非特异性蛋白的吸附或慢性炎症反应。然而，电荷掺入后观察到明显不同的细胞反应，带电荷的凝胶显着改善了3D3小鼠成纤维细胞在2D中的粘附力，并通过不需要全氟辛烷磺酸的“全合成”基质成功递送了存活且增殖的ARPE-19人视网膜上皮细胞。细胞粘附肽的掺入。