DNA nanostructures largely rely on pairing DNA bases; thus, sequence designing is required. Here, this study demonstrates a sequence‐independent strategy to fabricate DNA nanogel (NG) inspired by cisplatin, a chemotherapeutic drug that acts as a DNA crosslinker. A simple heating and cooling of the genomic DNA extracts and cisplatin produces DNA NG with a size controlled by the heating time. Furthermore, the drug‐loaded NG is formulated by spontaneously mixing DNA segments, cisplatin, and doxorubicin. The in vitro cell studies demonstrate that the doxorubicin‐loaded NG alters the drug distribution in cells while its cytotoxic potential is well‐maintained. This chemotherapeutic‐inspired method provides a facile one‐pot and cost‐effective strategy to fabricate size‐controllable DNA NG that potentially acts as drug carrier.

中文翻译：

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不依赖序列的DNA纳米凝胶作为潜在的药物载体

DNA纳米结构在很大程度上依赖于成对的DNA碱基。因此，需要进行序列设计。在这里，这项研究证明了顺铂（一种作为DNA交联剂的化学治疗药物）的启发，可以制造出一种与序列无关的策略来制造DNA纳米凝胶（NG）。简单加热和冷却基因组DNA提取物和顺铂可产生大小受加热时间控制的DNA NG。此外，通过自发混合DNA片段，顺铂和阿霉素来配制载药的NG。体外细胞研究表明，装载了阿霉素的NG可以很好地保持细胞毒性，从而改变药物在细胞中的分布。这种以化学疗法为灵感的方法提供了一种简便的，一站式且具有成本效益的策略，以制造可能用作药物载体的大小可控制的DNA NG。