Engineering scaffolds with excellent electro-activity is increasingly important in tissue engineering and regenerative medicine. Herein, conductive poly(3,4-ethylenedioxythiophene) doped with hyaluronic acid (PEDOT-HA) nanoparticles were firstly synthesized via chemical oxidant polymerization. A three-dimensional (3D) PEDOT-HA/Cs/Gel scaffold was then developed by introducing PEDOT-HA nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. HA, as a bridge, not only was used as a dopant, but also combined PEDOT into the Cs/Gel via chemical crosslinking. The PEDOT-HA/Cs/Gel scaffold was used as a conductive substrate for neural stem cell (NSC) culture in vitro. The results demonstrated that the PEDOT-HA/Cs/Gel scaffold had excellent biocompatibility for NSC proliferation and differentiation. 3D confocal fluorescence images showed cells attached on the channel surface of Cs/Gel and PEDOT-HA/Cs/Gel scaffolds with a normal neuronal morphology. Compared to the Cs/Gel scaffold, the PEDOT-HA/Cs/Gel scaffold not only promoted NSC proliferation with up-regulated expression of Ki67, but also enhanced NSC differentiation into neurons and astrocytes with up-regulated expression of β tubulin-III and GFAP, respectively. It is expected that this electro-active and bio-active PEDOT-HA/Cs/Gel scaffold will be used as a conductive platform to regulate NSC behavior for neural tissue engineering.

中文翻译：

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用于神经组织工程的导电性PEDOT-HA / Cs / Gel支架中神经干细胞的增殖和分化

具有优异电活性的工程支架在组织工程和再生医学中越来越重要。在此，首先通过化学氧化剂聚合合成了透明质酸（PEDOT-HA）纳米粒子掺杂的导电聚（3,4-乙撑二氧噻吩）。然后通过将PEDOT-HA纳米粒子引入壳聚糖/明胶（Cs / Gel）基质中来开发三维（3D）PEDOT-HA / Cs /凝胶支架。HA，作为桥，不仅用作掺杂剂，而且还通过化学交联将PEDOT结合到Cs / Gel中。PEDOT-HA / Cs / Gel支架用作神经干细胞（NSC）体外培养的导电底物。结果表明，PEDOT-HA / Cs / Gel支架对于NSC的增殖和分化具有极好的生物相容性。3D共聚焦荧光图像显示细胞附着在具有正常神经元形态的Cs / Gel和PEDOT-HA / Cs / Gel支架的通道表面上。与Cs / Gel支架相比，PEDOT-HA / Cs / Gel支架不仅可以通过上调Ki67的表达来促进NSC的增殖，还可以通过上调β-tubulin-III和Ns的表达来增强NSC向神经元和星形胶质细胞的分化。 GFAP。预期该电活性和生物活性的PEDOT-HA / Cs / Gel支架将用作调节神经组织工程中NSC行为的导电平台。