Nuclear movements are important for multiple cellular functions, and are driven by polarized forces generated by motor proteins and the cytoskeleton. During skeletal myofibre formation or regeneration, nuclei move from the centre to the periphery of the myofibre for proper muscle function. Centrally located nuclei are also found in different muscle disorders. Using theoretical and experimental approaches, we demonstrate that nuclear movement to the periphery of myofibres is mediated by centripetal forces around the nucleus. These forces arise from myofibril contraction and crosslinking that 'zip' around the nucleus in combination with tight regulation of nuclear stiffness by lamin A/C. In addition, an Arp2/3 complex containing Arpc5L together with γ-actin is required to organize desmin to crosslink myofibrils for nuclear movement. Our work reveals that centripetal forces exerted by myofibrils squeeze the nucleus to the periphery of myofibres.

中文翻译：

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肌原纤维收缩和交联驱动核运动到骨骼肌外围。

核运动对多种细胞功能很重要，并且由运动蛋白和细胞骨架产生的极化力驱动。在骨骼肌纤维形成或再生过程中，细胞核从肌纤维的中心移动到肌纤维的外围，以获得适当的肌肉功能。在不同的肌肉疾病中也发现了位于中央的细胞核。使用理论和实验方法，我们证明向肌纤维外围的核运动是由核周围的向心力介导的。这些力来自于肌原纤维收缩和交联，在细胞核周围“拉链”，以及核纤层蛋白 A/C 对核刚度的严格调节。此外，需要一个包含 Arpc5L 和 γ-肌动蛋白的 Arp2/3 复合物来组织结蛋白以交联肌原纤维以进行核运动。