AbstractThe authors describe a rapid and highly sensitive point-of-care device for rapid determination of noroviruses, a leading cause of acute gastroenteritis. The assay is based on the use of a norovirus-specific aptamer labeled with 6-carboxyfluorescein, and of multi-walled carbon nanotubes (MWCNT) and graphene oxide (GO). The fluorescence of the 6-FAM labeled aptamer is quenched by MWCNT or GO. In the presence of norovirus, fluorescence is recovered due to the release of the labeled aptamer from MWCNT or GO. An easy-to-make paper-based microfluidic platform was developed using a nitrocellulose membrane. The quantitation of norovirus was successfully performed. The linear range extends from 13 ng·mL−1 to 13 μg·mL−1 of norovirus. The detection limits are 4.4 ng·mL−1 and 3.3 ng·mL−1, respectively, when using MWCNT or GO. The device is simple and cost-effective, and holds the potential of rapid in-situ visual determination of noroviruses with remarkable sensitivity and specificity. Hence, it provides a new method for early identification of norovirus and a tool for early intervention when preventing the spread of an outbreak. Graphical Abstractᅟ

中文翻译：

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使用纸基微流体装置基于适体荧光测定诺如病毒

摘要作者描述了一种快速且高度灵敏的床旁设备，用于快速测定诺如病毒，这是导致急性肠胃炎的主要原因。该测定基于使用标有 6-羧基荧光素的诺如病毒特异性适体、多壁碳纳米管 (MWCNT) 和氧化石墨烯 (GO)。6-FAM 标记的适体的荧光被 MWCNT 或 GO 淬灭。在诺如病毒存在的情况下，由于从 MWCNT 或 GO 释放标记的适体，荧光被恢复。使用硝酸纤维素膜开发了一种易于制作的纸质微流体平台。诺如病毒定量成功。线性范围从 13 ng·mL-1 到 13 μg·mL-1 的诺如病毒。使用 MWCNT 或 GO 时，检测限分别为 4.4 ng·mL-1 和 3.3 ng·mL-1。该设备简单且具有成本效益，具有快速原位目测诺如病毒的潜力，具有显着的灵敏度和特异性。因此，它提供了一种早期识别诺如病毒的新方法和一种在防止疫情蔓延时进行早期干预的工具。图形摘要ᅟ