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Biosensing methods for determination of triglycerides: A review
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2017-09-09 , DOI: 10.1016/j.bios.2017.09.008
C.S. Pundir , Vinay Narwal

Triglycerides (TGs) are the major transporters of dietary fats throughout the bloodstream. Besides transporting fat, TGs also act as stored fat in adipose tissue, which is utilized during insufficient carbohydrates supply. TG level is below 150 mg/dL in healthy persons. Elevated TGs level in blood over 500 mg/dL is a biomarker for cardiovascular diseases, Alzheimer disease, pancreatitis and diabetes. Numerous methods are accessible for recognition of TGs, among them, most are cumbersome, time-consuming, require sample pre-treatment, high cost instrumental set-up and experienced personnel to operate. Biosensing approach overcomes these disadvantages, as these are highly specific, fast, easy, cost effective, and highly sensitive. This review article describes the classification, operating principles, merits and demerits of TG biosensors, specifically nanomaterials based biosensors. TG biosensors work ideally within 2.5–2700 s, in pH range, 6.0–11.0, temperature 25–39.5 °C and TG concentration range, 0.001–100 mM, the detection limits being in the range, 0.1 nM to 0.56 mM, with working potential − 0.02 to 1.2 V. These biosensors measured TG level in fruit juices, beverages, sera and urine samples and reused upto 200 times over a period of 7–240 days, while stored dry at 4 °C. Future perspective for further improvement and commercialization of TG biosensors are discussed.



中文翻译:

测定甘油三酸酯的生物传感方法

甘油三酸酯(TGs)是整个血液中膳食脂肪的主要转运体。除运输脂肪外,TGs还充当脂肪组织中储存的脂肪,在碳水化合物供应不足时会利用它们。健康人的TG水平低于150 mg / dL。血液中TGs升高至500 mg / dL以上是心血管疾病,阿尔茨海默氏病,胰腺炎和糖尿病的生物标志物。TG的识别方法很多,其中大多数繁琐,耗时,需要样品预处理,昂贵的仪器设置以及经验丰富的人员进行操作。生物传感方法克服了这些缺点,因为它们具有高度的特异性,快速性,简便性,成本效益和高度敏感性。这篇评论文章介绍了TG生物传感器的分类,工作原理,优缺点,特别是基于纳米材料的生物传感器。TG生物传感器在pH范围6.0–11.0,温度25–39.5°C和TG浓度范围0.001–100 mM的情况下,理想的工作时间为2.5–2700 s,工作范围为0.1 nM至0.56 mM电位-0.02至1.2V。这些生物传感器测量果汁,饮料,血清和尿液样品中的TG含量,并在7–240天的时间内重复使用多达200次,同时在4°C下干燥保存。讨论了TG生物传感器的进一步改进和商业化的未来前景。这些生物传感器可测量果汁,饮料,血清和尿液样本中的TG含量,并在7–240天的时间内重复使用多达200次,同时在4°C下干燥保存。讨论了TG生物传感器的进一步改进和商业化的未来前景。这些生物传感器可测量果汁,饮料,血清和尿液样本中的TG含量,并在7–240天的时间内重复使用多达200次,同时在4°C下干燥保存。讨论了TG生物传感器的进一步改进和商业化的未来前景。

更新日期:2017-09-09
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