Biomedical translational research has relied on two dimensional (2D) cell cultures for drug discovery over the decades, requiring cells to grow on a flat surface which does not always accurately model in vivo biological states. Three dimensional (3D) cell cultures, also known as 3D spheroids or organoids, grow as cellular tissues that are more physiologically relevant especially with respect to emulating cancer tumor-like structures [1]. While attractive, current methods for generating 3D spheroids has yet to replace 2D culturing methods used for drug discovery efforts that employ high-throughput screening (HTS), having limitations with scalability, reproducibility, and compatibility predominantly associated with conventional microtiter plate usage. Presented is a novel use of bead injection for the reproducible placement of spheroids and beads into high density microtiter plates of a 384- and 1536- well format.

数十年来，生物医学转化研究一直依赖于二维（2D）细胞培养物进行药物发现，这要求细胞在无法始终准确地模拟体内生物学状态的平坦表面上生长。三维（3D）细胞培养物（也称为3D球体或类器官）作为细胞组织生长，这些组织在生理上更相关，尤其是在模拟癌症肿瘤样结构方面[1]。尽管具有吸引力，但目前用于生成3D椭球体的方法尚未取代用于药物发现工作的2D培养方法，该方法采用高通量筛选（HTS），并且具有可扩展性，可再现性和兼容性方面的局限性，而这种局限性主要与常规微量滴定板的使用相关。