This study described the development and validation of a simple, rapid, specific and sensitive method for detecting chlormequat chloride (CQ) and mepiquat chloride (MQ) residues in tomato cultivation matrices covering soil, water, seedling samples. The dissipation rates of CQ and MQ in tomato cultivation matrices were also determined in this study. A Hydrophilic Interaction Liquid Chromatography (HILIC) column was used for chromatographic separation. A triple quadrupole mass spectrometer equipped with an electrospray ionisation source in positive ion mode by multiple reaction monitoring was used for detection. Soil samples were extracted with accelerated solvent extraction (ASE) and cleaned up with WCX phase extraction column; water samples were extracted with WCX phase extraction column; seedling samples were extracted with methanol-ammonium acetate solution. LODs and LOQs of CQ and MQ were 0.02 μg/kg and 0.1 μg/kg in soil samples, 0.005 ng/mL and 0.02 ng/mL in water samples, and 0.05 μg/kg and 1.0 μg/ kg in seedling samples, respectively. The mean recovery rate of CQ in soil, water and seedling samples ranged from 76.98% to 111.60%. While the mean recovery rate of MQ in soil, water and seedling samples ranged from 96.90% to 105.40%. The fastest to the slowest metabolising rates of CQ and MQ were as follows: soil samples > seedling samples > water samples. In conclusion, this study provided a new potential method for detecting CQ and MQ in tomato cultivation matrices using ultra-performance liquid chromatography-tandem mass spectrometry.

这项研究描述了一种简单，快速，特异和灵敏的方法的开发和验证，该方法可检测覆盖土壤，水和幼苗样品的番茄栽培基质中的氯甲基氯（CQ）和甲基异丙基氯（MQ）残留。这项研究还确定了CQ和MQ在番茄栽培基质中的耗散率。亲水相互作用液相色谱（HILIC）柱用于色谱分离。使用通过多重反应监测以正离子模式配备电喷雾电离源的三重四极杆质谱仪进行检测。用加速溶剂萃取（ASE）萃取土壤样品，并用WCX相萃取柱进行净化。用WCX相萃取柱萃取水样。用甲醇-乙酸铵溶液提取幼苗样品。CQ和MQ的LOD和LOQ在土壤样品中分别为0.02μg/ kg和0.1μg/ kg，在水样品中分别为0.005 ng / mL和0.02 ng / mL，在幼苗样品中分别为0.05μg/ kg和1.0μg/ kg。土壤，水和幼苗样品中CQ的平均回收率在76.98％至111.60％之间。土壤，水和幼苗样品中MQ的平均回收率在96.90％至105.40％之间。CQ和MQ的最快到最慢代谢速率如下：土壤样品>幼苗样品>水样品。总之，本研究提供了一种使用超高效液相色谱-串联质谱法检测番茄栽培基质中CQ和MQ的新方法。水样本中分别为005 ng / mL和0.02 ng / mL，幼苗样本中分别为0.05μg/ kg和1.0μg/ kg。土壤，水和幼苗样品中CQ的平均回收率在76.98％至111.60％之间。土壤，水和幼苗样品中MQ的平均回收率在96.90％至105.40％之间。CQ和MQ的最快到最慢代谢速率如下：土壤样品>幼苗样品>水样品。总之，本研究提供了一种使用超高效液相色谱-串联质谱法检测番茄栽培基质中CQ和MQ的新方法。水样本中分别为005 ng / mL和0.02 ng / mL，幼苗样本中分别为0.05μg/ kg和1.0μg/ kg。土壤，水和幼苗样品中CQ的平均回收率在76.98％至111.60％之间。土壤，水和幼苗样品中MQ的平均回收率在96.90％至105.40％之间。CQ和MQ的最快到最慢代谢速率如下：土壤样品>幼苗样品>水样品。总之，本研究提供了一种使用超高效液相色谱-串联质谱法检测番茄栽培基质中CQ和MQ的新方法。土壤，水和幼苗样品中MQ的平均回收率在96.90％至105.40％之间。CQ和MQ的最快到最慢代谢速率如下：土壤样品>幼苗样品>水样品。总之，本研究提供了一种使用超高效液相色谱-串联质谱法检测番茄栽培基质中CQ和MQ的新方法。土壤，水和幼苗样品中MQ的平均回收率在96.90％至105.40％之间。CQ和MQ的最快到最慢代谢速率如下：土壤样品>幼苗样品>水样品。总之，本研究提供了一种使用超高效液相色谱-串联质谱法检测番茄栽培基质中CQ和MQ的新方法。