Sugarcane honey (SCH) is one of the Madeira Island products par excellence and it is now popular worldwide. Its sui generis and peculiar sensory properties, explained by a variety of volatile compounds including furanic derivatives (FDs), arise mainly from manufacturing and storage conditions. A simple high-throughput approach based on semi-automatic microextraction by packed sorbent (MEPS) combined with ultra-high performance liquid chromatography (UHPLC) was developed and validated for identification and quantification of target FDs in sugarcane honey. A Quality-by-Design (QbD) approach was used as a powerful strategy to optimize analytical conditions for high throughput analysis of FDs in complex sugar-rich food matrices. The optimum point into MEPS-Method Operable Design: Region (MODR) was obtained with R-CX sorbent, acetonitrile (ACN) as elution solvent, three loading cycles and 500 μL of sample volume. The optimum point into UHPLC-MODR was obtained with a CORTECS column operating at a temperature of 50 °C, ACN as eluent and a flow rate of 125 μL min⁻¹. The robustness was demonstrated by Monte Carlo simulation and capability analysis for estimation of residual errors. The concentration-response relationship for all FDs were described by polynomial function models, being confirmed by Fisher variance (F-test). The% recoveries were in a range of 91.9-112.1%. Good method precision was observed, yielding relative standard deviations (RSDs) less than 4.9% for repeatability and 8.8% for intermediate precision. The limits of quantitation for the analytes ranged from 30.6 to 737.7 μg kg⁻¹. The MEPS_R-CX/UHPLC_CORTECS-PDA method revealed an effective and potential analytical tool for SCH authenticity control based on target analysis of FDs allowing a strict control and differentiation from other similar or adulterated products.

甘蔗蜂蜜（SCH）是马德拉岛卓越的产品之一，现已在全球范围内广受欢迎。它的特殊性包括呋喃衍生物（FDs）在内的多种挥发性化合物可以解释其特殊的感官特性，其主要源于制造和储存条件。建立了一种简单的高通量方法，该方法基于装填吸附剂（MEPS）与超高效液相色谱（UHPLC）结合的半自动微萃取，并已用于鉴定和定量甘蔗蜂蜜中的目标FD。设计质量（QbD）方法被用作一种强大的策略，可以优化复杂糖分高的食品基质中FD的高通量分析的分析条件。MEPS-方法可操作设计的最佳点：使用R-CX吸附剂，乙腈（ACN）作为洗脱溶剂，三个加载周期和500μL样品体积，获得了区域（MODR）。^-1。通过蒙特卡罗仿真和估计残差的能力分析证明了其鲁棒性。所有FD的浓度-响应关系均通过多项式函数模型描述，并通过Fisher方差（F检验）进行了确认。回收率百分比在91.9-112.1％的范围内。观察到良好的方法精度，相对标准偏差（RSD）的可重复性小于4.9％，中等精度的小于8.8％。分析物的定量限为30.6至737.7μgkg ^-1。MEPS _R-CX / UHPLC _CORTECS-PDA方法揭示了基于FD的目标分析进行SCH真实性控制的有效且潜在的分析工具，从而可以严格控制和区别于其他相似或掺假的产品。