Purification of extracts from Prangos haussknechtii Bioss afforded prenylated coumarins 1 and 2, monoterpenoid 3, amino acid derivative 4, and seven known compounds. Spectroscopic methods permitted establishment of the structures and relative configuration of these compounds. The pure isolates were tested for antioxidant and anti-inflammatory activities using lipid peroxidation (LPO), 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and cyclooxygenase (COX-1 and -2) enzyme inhibitory assays. Compounds 1–4 inhibited LPO with IC₅₀ values between 43 and 114 μM and reduced MTT to formazan blue between 48 and 128 μM. In anti-inflammatory assays using cyclooxygenase enzymes, COX-1 and -2, these compounds showed inhibition, with IC₅₀ values ranging from 34 to 56 μM.

中文翻译：

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脂蛋白的脂过氧化和环氧合酶抑制化合物

从Prangos haussknechtii Bioss提取物的纯化可得到烯丙基香豆素1和2，单萜3，氨基酸衍生物4和七个已知化合物。光谱方法允许建立这些化合物的结构和相对构型。使用脂质过氧化（LPO），3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）和环氧合酶（COX-1和-2）酶抑制试验。化合物1 - 4抑制LPO与IC ₅₀值介于43和114μM之间，MTT降低为48和128μM之间的甲蓝。在使用环氧合酶COX-1和-2进行的抗炎分析中，这些化合物表现出抑制作用，IC ₅₀值为34至56μM。