Characterizing the structures of glycoconjungates is important because of glycan heterogeneity and structural complexity of aglycon. The presence of relatively weak glycosidic linkages leads to preferential cleavages that limit the acquisition of structural information under typical mass spectrometry dissociation conditions, such as collision-induced dissociation (CID) and infrared multiphoton dissociation. In this paper, we explored the dissociation behaviors of different members of glycoconjugates, including glycopeptides, glycoalkaloids, and glycolipids, under electron-induced dissociation (EID) conditions. Using CID spectra as references, we found that EID is not only a complementary method to CID, but also a method that can generate extensive fragment ions for the structural characterization of all intact glycoconjugates studied. Furthermore, isomeric ganglioside species can be differentiated, and the double bond location in the ceramide moiety of the gangliosides can be identified through the MS³ approach involving sequential CID and EID processes.

中文翻译：

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使用电子诱导解离的串联质谱分析完整糖缀合物的结构表征

由于糖异质性和糖苷配基的结构复杂性，表征糖缀合物的结构很重要。相对较弱的糖苷键的存在会导致优先裂解，从而限制了在典型质谱解离条件下（例如碰撞诱导解离（CID）和红外多光子解离）的结构信息的获取。在本文中，我们探讨了在电子诱导解离（EID）条件下，糖缀合物的不同成员（包括糖肽，糖类生物碱和糖脂）的解离行为。使用CID光谱作为参考，我们发现EID不仅是CID的一种补充方法，还是一种可以生成大量碎片离子用于所有研究的完整糖缀合物的结构表征的方法。此外，涉及顺序CID和EID流程的^3种方法。