Acute graft-vs-host disease (GVHD) is a serious complication after allografting. We carried out an exploratory study to investigate a potential correlation of surface antigens on extracellular vesicles (EVs) and acute GVHD. EVs were extracted from serum samples from 41 multiple myeloma patients who underwent allografting. EVs were characterized by flow cytometry using a panel of 13 antibodies against specific membrane proteins that were reported to be predictive of acute GVHD. We observed a correlation between three potential biomarkers expressed on EV surface and acute GVHD onset by both logistic regression analysis and Cox proportional hazard model. In our study, CD146 (MCAM-1) was correlated with an increased risk-by almost 60%-of developing GVHD, whereas CD31 and CD140-α (PECAM-1 and PDGFR-α) with a decreased risk-by almost 40 and 60%, respectively. These biomarkers also showed a significant change in signal level from baseline to the onset of acute GVHD. Our novel study encourages future investigations into the potential correlation between EVs and acute GVHD. Larger prospective multicenter studies are currently in progress.

中文翻译：

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细胞外囊泡作为急性移植物抗宿主病的潜在生物标志物。

急性移植物抗宿主病（GVHD）是同种异体移植后的严重并发症。我们进行了一项探索性研究，以研究细胞外囊泡（EV）和急性GVHD上表面抗原的潜在相关性。EV是从41名接受同种异体移植的多发性骨髓瘤患者的血清样本中提取的。EV通过流式细胞术进行表征，使用了一组针对特定膜蛋白的13种抗体，这些抗体据报道可预测急性GVHD。我们通过逻辑回归分析和Cox比例风险模型观察了在EV表面表达的三种潜在生物标志物与急性GVHD发作之间的相关性。在我们的研究中，CD146（MCAM-1）与发展中的GVHD的风险增加了近60％相关，而CD31和CD140-α（PECAM-1和PDGFR-α）的风险下降了近40％。 60％分别。这些生物标志物还显示出从基线到急性GVHD发作之间信号水平的显着变化。我们的新颖研究鼓励对电动汽车与急性GVHD之间的潜在相关性进行进一步的研究。目前正在进行较大的前瞻性多中心研究。