当前位置: X-MOL 学术J. Proteome Res. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Therapeutic Mechanism Studies of ShuFengJieDu Capsule on an Acute Lung Injury Animal Model Using Quantitative Proteomics Technology
Journal of Proteome Research ( IF 4.4 ) Pub Date : 2017-09-07 00:00:00 , DOI: 10.1021/acs.jproteome.7b00409
Zhengang Tao , Xia Meng 1 , Yan-qi Han 2 , Ming-ming Xue , Shifei Wu 1 , Ping Wu 1 , Ying Yuan , Qiang Zhu 3 , Tie-Jun Zhang 2 , Catherine C. L. Wong 1
Affiliation  

ShuFengJieDu Capsule (SFJDC), a traditional Chinese Medicine (TCM) that comprises eight medicinal herbs, has been extensively utilized in the treatment of acute lung injury (ALI) and respiratory infections for more than 30 years in China. SFJDC has also been listed in the official guidelines of the CFDA (China Food and Drug Administration) because of its stabilized clinical manifestations. However, the underlying mechanism of SFJDC in repairing ALI remains unclear. In the present study, we explored the protective and therapeutic mechanism of SFJDC in a rat model using qualitative and quantitative proteomics. After establishing lipopolysaccharide (LPS)-induced ALI rat models, we profiled both isolated macrophage cells from freshly resected rat lung tissues from ALI models and section ALI rat lung tissues using a HPLC-MS/MS shotgun proteomics approach to identify changes in the levels of expression of proteins of interest. Based on the results of proteomics results and dysregulated protein analyses of ALI rat lung tissues and rat lung macrophages, AKT1 was selected as a putative key factor that might play an important role in the SFJDC treatment of ALI progression. Follow-up validation studies revealed that AKT1 expression effectively regulates various ALI-related molecules, and Gene Ontology analysis indicated that SFJDC-treated ALI rat macrophages were influenced by AKT1-based networks. Gain- and loss-of-function analyses via lentivirus-AKT1 or lentivirus-si-AKT1 infections into macrophages also indicated that AKT1 was essential for the development of ALI by regulating oxidative stress, apoptosis, or inflammatory responses. In summary, SFJDC effectively manipulated the biological activity in anti-inflammatory and immunomodulation activity in ALI, which might be involved in AKT1 regulation in ALI progression. New insights into SFJDC mechanisms may facilitate the development of novel pharmaceutical strategies in controlling the expression of inflammatory factors.
更新日期:2017-09-07
down
wechat
bug