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Melatonin improves the fertilization capacity and developmental ability of bovine oocytes by regulating cytoplasmic maturation events
Journal of Pineal Research ( IF 10.3 ) Pub Date : 2017-10-11 , DOI: 10.1111/jpi.12445
Xue-Ming Zhao 1 , Na Wang 1 , Hai-Sheng Hao 1 , Chong-Yang Li 1 , Ya-Han Zhao 1 , Chang-Liang Yan 2 , Hao-Yu Wang 1 , Wei-Hua Du 1 , Dong Wang 1 , Yan Liu 1 , Yun-Wei Pang 1 , Hua-Bin Zhu 1
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Melatonin is a well‐characterized antioxidant that has been successfully used to protect oocytes from reactive oxygen species during in vitro maturation (IVM), resulting in improved fertilization capacity and development ability. However, the mechanism via which melatonin improves oocyte fertilization capacity and development ability remains to be determined. Here, we studied the effects of melatonin on cytoplasmic maturation of bovine oocytes. In the present study, bovine oocytes were cultured in IVM medium supplemented with 0, 10–7, 10–9, and 10−11 mol/L melatonin, and the cytoplasmic maturation parameters of MII oocytes after IVM were investigated, including redistribution of organelles (mitochondria, cortical granules [CGs], and endoplasmic reticulum [ER]), intracellular glutathione (GSH) and ATP levels, expression of endogenous antioxidant genes (Cat, Sod1, and GPx), and fertilization‐related events (IP3R1 distribution and expression of CD9 and Juno). Our results showed that melatonin significantly improved the cytoplasmic maturation of bovine oocytes by improving the normal distribution of organelles, increasing intracellular GSH and ATP levels, enhancing antioxidant gene expression levels, and modulating fertilization‐related events, all of which resulted in increased fertilization capacity and developmental ability. Meanwhile, melatonin also increased the mRNA and protein expression levels of the Tet1 gene and decreased the Dnmt1 gene mRNA and protein levels in bovine oocytes, indicating that melatonin regulates the expression of the detected genes via demethylation. These findings shed insights into the potential mechanisms by which melatonin improves oocyte quality during IVM.

中文翻译:

褪黑素通过调节细胞质成熟事件来提高牛卵母细胞的受精能力和发育能力

褪黑激素是一种具有良好特征的抗氧化剂,已成功用于保护卵母细胞免受体外成熟(IVM)过程中的活性氧的影响,从而提高了受精能力和发育能力。然而,褪黑激素改善卵母细胞受精能力和发育能力的机制尚待确定。在这里,我们研究了褪黑激素对牛卵母细胞细胞质成熟的影响。在本研究中,牛卵母细胞在补充有0 IVM培养基,10℃培养-7,10 -9,10 -11 研究了mol / L褪黑素以及IVM后MII卵母细胞的细胞质成熟参数,包括细胞器(线粒体,皮质颗粒[CG]和内质网[ER])的重新分布,细胞内谷胱甘肽(GSH)和ATP水平,内源性抗氧化剂基因(CatSod1GPx)以及受精相关事件(IP3R1分布以及CD9和Juno的表达)。我们的研究结果表明,褪黑激素可通过改善细胞器的正常分布,增加细胞内GSH和ATP水平,增强抗氧化剂基因表达水平以及调节受精相关事件来显着改善牛卵母细胞的细胞质成熟,所有这些均导致受精能力增强和发展能力。同时,褪黑激素还增加了Tet1基因的mRNA和蛋白质表达水平,并降低了Dnmt1牛卵母细胞中的基因mRNA和蛋白水平,表明褪黑激素通过去甲基化调节检测到的基因的表达。这些发现为褪黑激素改善IVM期间卵母细胞质量的潜在机制提供了见识。
更新日期:2017-10-11
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