当前位置: X-MOL 学术Trends Mol. Med. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
De Novo Gene Expression Reconstruction in Space
Trends in Molecular Medicine ( IF 13.6 ) Pub Date : 2017-05-29 , DOI: 10.1016/j.molmed.2017.05.004
Je H Lee 1
Affiliation  

The biological function of a gene often depends on spatial context, and an atlas of transcriptional regulation could be instrumental in defining functional elements across the genome. Despite recent advances in single-cell RNA sequencing and in situ RNA imaging, fundamental barriers limit the speed, genome-wide coverage, and resolution of de novo transcriptome assembly in space. Here, I discuss potential next-generation approaches for the de novo assembly of the transcriptome in space, and propose more efficient methods of detecting long-range spatial variations in gene expression. Finally, I discuss future in situ sequencing chemistries for visualizing biological pathways and processes in tissues so that genomics technologies might be more easily applied to conditions of human health and disease.



中文翻译:

空间从头基因表达重建

基因的生物学功能通常取决于空间背景,转录调控图谱可能有助于定义整个基因组的功能元件。尽管单细胞 RNA 测序和原位RNA 成像最近取得了进展,但基本障碍限制了空间从头转录组组装的速度、全基因组覆盖范围和分辨率。在这里,我讨论了在空间中从头组装转录组的潜在下一代方法,并提出了检测基因表达的远程空间变化的更有效的方法。最后,我讨论了未来的原位测序化学,用于可视化组织中的生物途径和过程,以便基因组学技术可以更容易地应用于人类健康和疾病条件。

更新日期:2017-05-29
down
wechat
bug