Interfacing mass spectrometry (MS) with microfluidic chips (μchip-MS) holds considerable potential to transform a clinician’s toolbox, providing translatable methods for the early detection, diagnosis, monitoring, and treatment of noncommunicable diseases by streamlining and integrating laborious sample preparation workflows on high-throughput, user-friendly platforms. Overcoming the limitations of competitive immunoassays − currently the gold standard in clinical proteomics − μchip-MS can provide unprecedented access to complex proteomic assays having high sensitivity and specificity, but without the labor, costs, and complexities associated with conventional MS sample processing. This review surveys recent μchip-MS systems for clinical applications and examines their emerging role in streamlining the development and translation of MS-based proteomic assays by alleviating many of the challenges that currently inhibit widespread clinical adoption.

质谱（MS）与微流控芯片（μchip-MS）的连接具有巨大的潜力来转变临床医生的工具箱，通过简化和整合繁琐的高效率样品制备工作流程，为非传染性疾病的早期检测，诊断，监测和治疗提供可翻译的方法通量，用户友好的平台。克服竞争性免疫测定法的局限性（目前是临床蛋白质组学的金标准），μchip-MS可以空前地获得具有高灵敏度和特异性的复杂蛋白质组学测定法，而无需人工，成本和与常规MS样品处理相关的复杂性。